Analysis of terizidone in plasma using HPLC‐UV method and its application in a pharmacokinetic study of patients with drug‐resistant tuberculosis

A chromatographic method has been developed and validated for the first time for analysis of terizidone in plasma. Terizidone was extracted from plasma by protein precipitation using a mixture of acetonitrile and methanol (1:1, v/v). The chromatographic separation was achieved with a gradient of ace...

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Bibliographic Details
Published in:Biomedical chromatography 2018-11, Vol.32 (11), p.e4325-n/a
Main Authors: Mulubwa, Mwila, Mugabo, Pierre
Format: Article
Language:English
Subjects:
HPLC‐UV
multidrug‐resistant tuberculosis
plasma
Terizidone
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Summary:A chromatographic method has been developed and validated for the first time for analysis of terizidone in plasma. Terizidone was extracted from plasma by protein precipitation using a mixture of acetonitrile and methanol (1:1, v/v). The chromatographic separation was achieved with a gradient of acetonitrile and water both containing 0.1% formic acid on a Supelco Discovery® HS C18 (150 × 4.6 mm, 5 μm) reversed‐phase column. Propranolol was used as the internal standard. The total run‐time was 18 min. The calibration standard concentrations ranged between 3.125 and 200 μg/mL and calibration curves were linear with coefficient of determination values in the range of 0.9988–0.9999. The inter‐ and intra‐day assay precision (percentage relative standard error) was
ISSN:0269-3879
1099-0801
DOI:10.1002/bmc.4325