Direct identification of non-polio enteroviruses in residual paralysis cases by analysis of VP sub(1) sequences

• Published in: Journal of clinical virology 2009-06, Vol.45 (2), p.139-141
• Main Authors: Rahimi, P, Tabatabaie, H, Gouya, M M, Mahmudi, M, Musavi, T, Samimi Rad, K, Mokhtari Azad, T, Nategh, R
• Format: Article
• Language: English
• Subjects: Enterovirus; Poliovirus
• ISSN: 1386-6532
• DOI: 10.1016/j.jcv.2009.03.004

Background: The 66 serotypes of human enteroviruses (EVs) are classified into four species A-D, based on phylogenetic relationships in multiple genome regions. Partial VP sub(1) amplification and sequence analysis are reliable methods for identifying non-polio enterovirus serotypes, especially in negative cell culture specimens from patients with residual paralysis. Objectives: In Iran during the years 2000-2002, there were 29 residual paralysis cases with negative cell (RD, HEp sub(2) and L sub(2) sub(0)B) culture results. Study design: The genomic RNA was extracted from stool specimens from cases of residual paralysis and detected by amplification of the 5'-nontranslated region using RT-PCR with Pan-EV primers. Partial VP sub(1) amplification by semi-nested RT-PCR (snRT-PCR) and sequence analysis were done. Results: Specimens from the 29 culture-negative cases contained echoviruses of six different serotypes. Conclusions: The global eradication of wild polioviruses is near and study of non-polio enteroviruses, which can cause poliomyelitis, is increasingly important to understand their pathogenesis. The VP sub(1) sequences, derived from the snRT-PCR products, allowed rapid molecular analysis of these non-polio strains.