Nanoquencher‐Based Selective Imaging of Protein Glutathionylation in Live Mammalian Cells

Changes in the cellular levels of glutathione (GSH) and protein S‐glutathionylation (PSSG) are closely associated with a number of human diseases. Despite recent advances, few thiol‐reactive, small‐molecule GSH sensors could selectively detect GSH over other endogenous thiols, and none was capable o...

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Bibliographic Details
Published in:Angewandte Chemie International Edition 2018-08, Vol.57 (32), p.10257-10262
Main Authors: Mao, Xin, Yuan, Peiyan, Yu, Changmin, Li, Lin, Yao, Shao Q.
Format: Article
Language:English
Subjects:
antibodies
biosensors
Fluorescence
Glutathione
Glutathione - chemistry
Glutathione - metabolism
glutathionylated proteins
HeLa Cells
Humans
imaging
Mammalian cells
Mammals
mesoporous silica nanoquenchers
Nanoparticles - chemistry
Optical Imaging
Protein S
Protein S - chemistry
Protein S - metabolism
Proteins
Selective binding
Silica
Silicon dioxide
Silicon Dioxide - chemistry
Thiols
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Summary:Changes in the cellular levels of glutathione (GSH) and protein S‐glutathionylation (PSSG) are closely associated with a number of human diseases. Despite recent advances, few thiol‐reactive, small‐molecule GSH sensors could selectively detect GSH over other endogenous thiols, and none was capable of detecting PSSG in live mammalian cells. By using a dye‐loaded mesoporous silica nanoquencher (qMSN) capped with anti‐GSH antibody capable of highly selective binding toward GSH and glutathionylated proteins over other molecules, we have successfully developed a fluorescence GSH/PSSG nanosensor, which showed unprecedented selectivity toward PSSG even in the presence of GSH, had amplifiable and programmable fluorescence Turn‐ON properties, and could be used to image endogenous PSSG in live mammalian cells under stimulated conditions for the first time. Selective detection: By using a mesoporous silica nanoquencher capped with anti‐glutathione‐S‐transferase antibody, a fluorescence nanosensor has been developed. The nanosensor is capable of selectively imaging endogenous protein glutathionylation from live mammalian cells under stimulated conditions.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201806710