Thermoactivity and effects of organic solvents on digestive lipase from hepatopancreas of the green crab

Unlike classical digestive lipases, the crab digestive lipase (CDL) displayed its maximal activity at a high temperature. The CDL activity’s optimal temperature, when using emulsified or monomolecular film as substrate, was 60 °C. To our knowledge, this is the first report of an animal digestive lip...

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Bibliographic Details
Published in:Food chemistry 2009-09, Vol.116 (1), p.82-86
Main Authors: Cherif, Slim, Gargouri, Youssef
Format: Article
Language:English
Subjects:
Biological and medical sciences
Carcinus maenas
Characterisation
Crab digestive lipase
Decapoda
enzyme activity
Fish and seafood industries
food analysis
food biotechnology
food composition
Food industries
Fundamental and applied biological sciences. Psychology
Hepatopancreas
Organic solvent
organic solvents
solvents
Stability
thermal properties
thermoactivity
triacylglycerol lipase
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Summary:Unlike classical digestive lipases, the crab digestive lipase (CDL) displayed its maximal activity at a high temperature. The CDL activity’s optimal temperature, when using emulsified or monomolecular film as substrate, was 60 °C. To our knowledge, this is the first report of an animal digestive lipase having such an optimal temperature. The maximum activity of CDL appeared at pH 8. Lipase activity was compatible with the presence of organic solvents, except for butanol. Furthermore, the hydrolysis was found to be specifically dependent on the presence of Ca 2+ ions, since no significant CDL activity was detected in the presence of ion chelators such as EDTA. Nevertheless, the CDL does not require Ca 2+ to trigger the hydrolysis of tributyrin emulsion. Interestingly, Zn 2+ and Cu 2+ ions acted as strong inhibitors of CDL activity when using tributyrin as substrate. Lipase stability in the presence of organic solvents, as well as at high temperatures, makes it a good candidate for application in non-aqueous catalysis.
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2009.02.009