Investigation of Yersinia pestis and Yersinia pseudotuberculosis strains from Georgia and neighboring countries in the Caucasus by high-density SNP microarray

Yersinia pestis , the causative agent of plague, is a recently evolved clone of the enteropathogenic bacterium Yersinia pseudotuberculosis. Y. pestis has been extensively studied for decades; however, there are insufficient data about the intra-species diversity of this microorganism in certain part...

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Bibliographic Details
Published in:Archives of microbiology 2018-11, Vol.200 (9), p.1345-1355
Main Authors: Zhgenti, Ekaterine, Hu, Ping, Chanturia, Gvantsa, Tsereteli, David, Kekelidze, Merab, Chubinidze, Svetlana, Zangaladze, Ekaterine, Imnadze, Paata, Andersen, Gary, Torok, Tamas
Format: Article
Language:English
Subjects:
Biochemistry
Biodiversity
Biomedical and Life Sciences
Biotechnology
Cell Biology
Density
Ecology
Genomes
Genotype
Genotyping
Genotyping Techniques
Georgia (Republic) - epidemiology
Life Sciences
Microbial Ecology
Microbiology
Original Paper
Pandemics
Pathogens
Phylogeny
Plague
Plague - epidemiology
Plague - microbiology
Plasmids
Plasmids - genetics
Polymorphism
Polymorphism, Single Nucleotide - genetics
Pseudotuberculosis
Reproducibility
Reproducibility of Results
Single-nucleotide polymorphism
Species diversity
Yersinia pestis
Yersinia pestis - genetics
Yersinia pestis - isolation & purification
Yersinia pseudotuberculosis
Yersinia pseudotuberculosis - genetics
Yersinia pseudotuberculosis - isolation & purification
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Summary:Yersinia pestis , the causative agent of plague, is a recently evolved clone of the enteropathogenic bacterium Yersinia pseudotuberculosis. Y. pestis has been extensively studied for decades; however, there are insufficient data about the intra-species diversity of this microorganism in certain parts of the world, including the Caucasus region. Using a high-density single-nucleotide polymorphism (SNP) microarray, we genotyped a total of 46 Y. pestis isolates from two plague foci in Georgia and neighboring Caucasus countries together with 12 Y. pseudotuberculosis isolates from Georgia. The genotyping microarray captured a total of 13,525 SNP positions across the Y. pestis and Y. pseudotuberculosis genomes and plasmids with high-throughput capability and superior reproducibility. From this analysis, we confirmed the presence of two independent and relatively distant phylogenetic groups of Y. pestis in the Caucasus region. The signature SNP patterns identified from this study will allow assay development for plague surveillance and pseudotuberculosis diagnostics.
ISSN:0302-8933
1432-072X
DOI:10.1007/s00203-018-1545-8