Gut–liver on a chip toward an in vitro model of hepatic steatosis

Hepatic steatosis is a process of abnormal lipid deposition within the liver cells, often caused by excessive alcohol uptake or obesity. A conventional in vitro model for hepatic steatosis uses a liver cell culture, treated with fatty acids and measures accumulation of lipids within the cells. This...

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Bibliographic Details
Published in:Biotechnology and bioengineering 2018-11, Vol.115 (11), p.2817-2827
Main Authors: Lee, Seung Yeon, Sung, Jong Hwan
Format: Article
Language:English
Subjects:
Absorption
Accumulation
Alcohols
Butyrates - metabolism
Caco-2 Cells
Cell culture
Deposition
Digestive system
Fatty acids
Fatty liver
Fatty Liver - pathology
Gastrointestinal tract
Gastrointestinal Tract - pathology
gut–liver chip
Hep G2 Cells
hepatic steatosis
Hepatocytes
Humans
Lab-On-A-Chip Devices
Lipid Metabolism
Lipids
Lipoic acid
Liver
Metabolism
Microfluidics
Microfluidics - instrumentation
Microfluidics - methods
organ‐on‐a‐chip
Steatosis
Thioctic Acid - metabolism
Tumor Necrosis Factor-alpha - metabolism
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Summary:Hepatic steatosis is a process of abnormal lipid deposition within the liver cells, often caused by excessive alcohol uptake or obesity. A conventional in vitro model for hepatic steatosis uses a liver cell culture, treated with fatty acids and measures accumulation of lipids within the cells. This model does not recapitulate the complex process of absorption and metabolism of digestive lipids. Here, we introduce a gut–liver chip, which mimics the gut absorption and hepatic metabolism in a microfluidic chip. Absorption of fatty acids through gut layer and subsequent deposition within liver cells was demonstrated. Tumor necrosis factor‐α, butyrate, and α‐lipoic acid were chosen as model molecules that can affect hepatic steatosis via different mechanisms, and their effects were evaluated. Our results suggest that the gut–liver chip can mimic the absorption and accumulation of fatty acids in the gut and the liver. Hepatic steatosis is a process of abnormal lipid deposition within the liver cells, often caused by excessive alcohol uptake or obesity. A conventional in vitro model for hepatic steatosis uses a liver cell culture, treated with fatty acids and measures accumulation of lipids within the cells.
ISSN:0006-3592
1097-0290
DOI:10.1002/bit.26793