Dopamine transporter function differences between male and female CD-1 mice

It has been reported that male mice are more susceptible to the neurotoxic effects of methamphetamine (MA) upon the nigrostriatal dopaminergic (NSDA) system. Since MA utilizes the dopamine transporter (DAT) to exert its effects, in the present study, we tested for differences in the dynamics of DAT...

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Bibliographic Details
Published in:Brain research 2005-02, Vol.1035 (2), p.188-195
Main Authors: Bhatt, Sandeep D., Dluzen, Dean E.
Format: Article
Language:English
Subjects:
3,4-Dihydroxyphenylacetic Acid - metabolism
Animals
Biological and medical sciences
Corpus Striatum - physiology
Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases
Dopamine - physiology
Dopamine Plasma Membrane Transport Proteins
Female
Male
Medical sciences
Membrane Glycoproteins - physiology
Membrane Transport Proteins - physiology
Methamphetamine
Mice
Nerve Tissue Proteins - physiology
Nervous system (semeiology, syndromes)
Nervous system as a whole
Neurology
Neuroprotection
Neurotoxicity
Nomifensine
Parkinson's disease
Sex Characteristics
Sex differences
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Summary:It has been reported that male mice are more susceptible to the neurotoxic effects of methamphetamine (MA) upon the nigrostriatal dopaminergic (NSDA) system. Since MA utilizes the dopamine transporter (DAT) to exert its effects, in the present study, we tested for differences in the dynamics of DAT function between male and female mice as an approach to understand some of the bases for this sex difference in MA-induced NSDA neurotoxicity. To accomplish this goal, in Experiment 1, the amount of dopamine (DA) obtained following DA infusion into the superfused striatal tissue fragments of male and female mice was measured while in Experiment 2 responses to the DA uptake blocker, nomifensine (NMF), were assessed in these preparations. The differences obtained to these treatments demonstrate that marked differences in DA transporter activity exist between male and female mice. When combining the DA and DOPAC measures from these two experiments, the data suggest that the female mice show a more active and efficient recovery and vesicular packaging of extracellular DA. These findings have important implications for sex differences in NSDA functions and responses to neurotoxins which enter the neurons via the DAT.
ISSN:0006-8993
1872-6240
DOI:10.1016/j.brainres.2004.12.013