A fluorescent chemical probe CDy9 selectively stains and enables the isolation of live naïve mouse embryonic stem cells

Human and mouse embryonic stem cells (ESCs) differ in terms of their pluripotency status, i.e., naïve vs. primed. This affects various biological properties and leads to several technical hurdles for future clinical applications, such as difficulties in chimera formation, single-cell passaging, and...

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Bibliographic Details
Published in:Biomaterials 2018-10, Vol.180, p.12-23
Main Authors: Cho, Seung-Ju, Kim, Keun-Tae, Kim, Jong-Soo, Kwon, Ok-Seon, Go, Young-Hyun, Kang, Nam-Young, Heo, Haejeong, Kim, Mi-Rang, Han, Dong Wook, Moon, Sung-Hwan, Chang, Young-Tae, Cha, Hyuk-Jin
Format: Article
Language:English
Subjects:
Fluorescence probe
Inner cell mass
Live isolation
Naïve ESCs
Primed ESCs
Slc13a5
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Summary:Human and mouse embryonic stem cells (ESCs) differ in terms of their pluripotency status, i.e., naïve vs. primed. This affects various biological properties and leads to several technical hurdles for future clinical applications, such as difficulties in chimera formation, single-cell passaging, and gene editing. In terms of generating functional human tissues and organs via mammalian interspecies chimerism, a fluorescent chemical probe that specifically labels naïve ESCs would help to isolate these cells and monitor their conversion. This study demonstrates that the fluorescent chemical probe compound of designation yellow 9 (CDy9) selectively stains naïve, but not primed, mouse ESCs (mESCs). CDy9 entered cells via Slc13a5, a highly expressed membrane transporter in naïve mESCs. Fluorescence-based cell sorting based on CDy9 staining successfully separated naïve mESCs from primed mESCs. Mice generated using CDy9+ cells isolated during the conversion of mouse epiblast stem cells into naïve mESCs exhibited coat color chimerism. Furthermore, CDy9 specifically stained cells in the inner cell mass of mouse embryos. These findings suggest that CDy9 is a useful tool to isolate functional naïve mESCs.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2018.07.007