Using FT-IR spectroscopy for the identification of the T. cruzi, T. rangeli, and the L. chagasi species

The cross-reaction in the diagnosis results is a serious problem, leading to an incorrect treatment and several injuries to patients. The Trypanosoma rangeli and Trypanosoma cruzi belong to the genus Trypanosoma, but the Trypanosoma rangeli is a non-pathogenic parasite to humans. While Trypanosoma c...

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Bibliographic Details
Published in:Experimental parasitology 2018-09, Vol.192, p.46-51
Main Authors: Aguiar, J.C., Mittmann, J., Caetano, P.C., Raniero, L.
Format: Article
Language:English
Subjects:
Biochemistry
FT-IR spectroscopy
Leishmania chagasi
Trypanosoma cruzi
Trypanosoma rangeli
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Summary:The cross-reaction in the diagnosis results is a serious problem, leading to an incorrect treatment and several injuries to patients. The Trypanosoma rangeli and Trypanosoma cruzi belong to the genus Trypanosoma, but the Trypanosoma rangeli is a non-pathogenic parasite to humans. While Trypanosoma cruzi is the etiological agent of Chagas' disease, which affects circa 2–3 million people and more than 6000 deaths annually in Brazil. The Leishmania chagasi causes infectious disease known as visceral leishmaniasis. This diseases have in common the crossed antigenic reaction promoted by serological tests and its differentiation is relevant for epidemiological studies and clinical practice. In this study the Fourier Transform Infrared (FT-IR) Spectroscopy was used to differentiate these microorganisms, which were cultivated and the spectra analyzed. Data analysis were performed by Gaussian curve fitting and multivariate statistical analysis. The cluster analysis have shown four specific regions to identify the microorganisms. The first three PCs of principal component analysis associated to linear discriminant were able to classify 95.6% of the parasites using cross-validation. The curve fitting method showed the quantitative differentiation among L. chagasi, T. cruzi, and T. rangeli species in the vibrational regions of polysaccharides, amide III, lipid esters, and fatty acid. [Display omitted] •Differentiation of T. cruzi, T. rangeli, and L. chagasi species by FTIR.•Four spectral regions allowed identifying these parasites by Cluster analysis.•Band deconvolution of the spectral regions that separate the parasites.
ISSN:0014-4894
1090-2449
DOI:10.1016/j.exppara.2018.07.008