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Chlorogenic acids inhibit glutamate dehydrogenase and decrease intracellular ATP levels in cultures of chick embryo retina cells

[Display omitted] Chlorogenic acids (CGAs) are a group of phenolic compounds found in worldwide consumed beverages such as coffee and green tea. They are synthesized from an esterification reaction between cinnamic acids, including caffeic (CFA), ferulic and p-coumaric acids with quinic acid (QA), f...

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Published in:Biochemical pharmacology 2018-09, Vol.155, p.393-402
Main Authors: Domith, Ivan, Duarte-Silva, Aline T., Garcia, Carlos Gustavo, Calaza, Karin da Costa, Paes-de-Carvalho, Roberto, Cossenza, Marcelo
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container_title Biochemical pharmacology
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creator Domith, Ivan
Duarte-Silva, Aline T.
Garcia, Carlos Gustavo
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Cossenza, Marcelo
description [Display omitted] Chlorogenic acids (CGAs) are a group of phenolic compounds found in worldwide consumed beverages such as coffee and green tea. They are synthesized from an esterification reaction between cinnamic acids, including caffeic (CFA), ferulic and p-coumaric acids with quinic acid (QA), forming several mono- and di-esterified isomers. The most prevalent and studied compounds are 3-O-caffeoylquinic acid (3-CQA), 4-O-caffeoylquinic acid (4-CQA) and 5-O-caffeoylquinic acid (5-CQA), widely described as having antioxidant and cell protection effects. CGAs can also modulate glutamate release from microglia by a mechanism involving a decrease of reactive oxygen species (ROS). Increased energy metabolism is highly associated with enhancement of ROS production and cellular damage. Glutamate can also be used as an energy source by glutamate dehydrogenase (GDH) enzyme, providing α-ketoglutarate to the tricarboxylic acid (TCA) cycle for ATP synthesis. High GDH activity is associated with some disorders, such as schizophrenia and hyperinsulinemia/hyperammonemia syndrome. In line with this, our objective was to investigate the effect of CGAs on GDH activity. We show that CGAs and CFA inhibits GDH activity in dose-dependent manner, reaching complete inhibition at high concentration with IC50 of 52 μM for 3-CQA and 158.2 μM for CFA. Using live imaging confocal microscopy and microplate reader, we observed that 3-CQA and CFA can be transported into neuronal cells by an Na+-dependent mechanism. Moreover, neuronal cells treated with CGAs presented lower intracellular ATP levels. Overall, these data suggest that CGAs have therapeutic potential for treatment of disorders associated with high GDH activity.
doi_str_mv 10.1016/j.bcp.2018.07.023
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subjects Adenosine Triphosphate - antagonists & inhibitors
Adenosine Triphosphate - metabolism
Animals
Caffeic acid
Cells, Cultured
Chick Embryo
Chlorogenic Acid - pharmacology
Dose-Response Relationship, Drug
Fluorescence uptake
Glutamate Dehydrogenase - antagonists & inhibitors
Glutamate Dehydrogenase - metabolism
Intracellular Fluid - drug effects
Intracellular Fluid - metabolism
Neuronal and glial metabolism
Quinic acid
Retina - cytology
Retina - drug effects
Retina - metabolism
title Chlorogenic acids inhibit glutamate dehydrogenase and decrease intracellular ATP levels in cultures of chick embryo retina cells
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