Inter-simple sequence repeat fingerprints to assess genetic diversity in Tunisian fig (Ficus carica L.) germplasm

The genetic diversity of 18 Tunisian fig cultivars was investigated at the DNA level using the Inter Simple Sequence Repeat (ISSR) associated with the Polymerase Chain Reaction (PCR). Using a set of primers, the most informative ones were selected that were characterized by an important Resolving po...

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Bibliographic Details
Published in:Genetic resources and crop evolution 2004-05, Vol.51 (3), p.269-275
Main Authors: Amel, S.H, Mokhtar, T, Salwa, Z, Jihene, H, Messaoud, M, Abdelmajid, R, Mohamed, M
Format: Article
Language:English
Subjects:
Cultivars
Deoxyribonucleic acid
DNA
DNA fingerprinting
Fingerprinting
Fragments
Gene polymorphism
Genetic diversity
Genetic relationship
Germplasm
Markers
Nucleotide sequence
Polymerase chain reaction
Polymorphism
Primers
Principal components analysis
Resolution
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Summary:The genetic diversity of 18 Tunisian fig cultivars was investigated at the DNA level using the Inter Simple Sequence Repeat (ISSR) associated with the Polymerase Chain Reaction (PCR). Using a set of primers, the most informative ones were selected that were characterized by an important Resolving power value of 29.6. A total of 47 discernible fragments were scored from samples, with a mean of 11.7 fragments per primer. The 90.4% of sample that were polymorphic were scored as molecular markers to examine the Tunisian fig germplasm polymorphism at DNA level. A large genetic diversity as related to ISSR patterns was found within the local Tunisian fig germplasm. An UPGMA dendrogram exhibits the unstructured variability in this crop. Moreover, the principal component analysis shows that the observed diversity was typically continuous. Our data provide a large number of ISSR markers that are useful in the fingerprinting of Ficuscarica L. cultivars, and in the understanding of the genetic relationships among these accessions.
ISSN:0925-9864
1573-5109
DOI:10.1023/B:GRES.0000024012.59828.6b