Inter-simple sequence repeat fingerprints to assess genetic diversity in Tunisian fig (Ficus carica L.) germplasm

• Published in: Genetic resources and crop evolution 2004-05, Vol.51 (3), p.269-275
• Main Authors: Amel, S.H, Mokhtar, T, Salwa, Z, Jihene, H, Messaoud, M, Abdelmajid, R, Mohamed, M
• Format: Article
• Language: English
• Subjects: Cultivars; Deoxyribonucleic acid; DNA; DNA fingerprinting; Fingerprinting; Fragments; Gene polymorphism; Genetic diversity; Genetic relationship; Germplasm; Markers; Nucleotide sequence; Polymerase chain reaction; Polymorphism; Primers; Principal components analysis; Resolution
• ISSN: 0925-9864; 1573-5109
• DOI: 10.1023/B:GRES.0000024012.59828.6b

The genetic diversity of 18 Tunisian fig cultivars was investigated at the DNA level using the Inter Simple Sequence Repeat (ISSR) associated with the Polymerase Chain Reaction (PCR). Using a set of primers, the most informative ones were selected that were characterized by an important Resolving power value of 29.6. A total of 47 discernible fragments were scored from samples, with a mean of 11.7 fragments per primer. The 90.4% of sample that were polymorphic were scored as molecular markers to examine the Tunisian fig germplasm polymorphism at DNA level. A large genetic diversity as related to ISSR patterns was found within the local Tunisian fig germplasm. An UPGMA dendrogram exhibits the unstructured variability in this crop. Moreover, the principal component analysis shows that the observed diversity was typically continuous. Our data provide a large number of ISSR markers that are useful in the fingerprinting of Ficuscarica L. cultivars, and in the understanding of the genetic relationships among these accessions.