Mancozeb exposure results in manganese accumulation and Nrf2-related antioxidant responses in the brain of common carp Cyprinus carpio

• Published in: Environmental science and pollution research international 2018-06, Vol.25 (16), p.15529-15540
• Main Authors: Costa-Silva, Dennis Guilherme, Lopes, Andressa Rubim, Martins, Illana Kemmerich, Leandro, Luana Paganotto, Nunes, Mauro Eugênio Medina, de Carvalho, Nelson Rodrigues, Rodrigues, Nathane Rosa, Macedo, Giulianna Echeveria, Saidelles, Ana Paula, Aguiar, Cassiana, Doneda, Morgana, Flores, Erico Marlon Moraes, Posser, Thais, Franco, Jeferson Luis
• Format: Article
• Language: English
• Subjects: Activation; Animals; Antioxidants; Antioxidants - metabolism; Aquatic organisms; Aquatic Pollution; Atmospheric Protection/Air Quality Control/Air Pollution; Bioaccumulation; Blood; Brain; Brain - drug effects; Brain - metabolism; Carp; Carps - metabolism; Cyprinidae; Cyprinus carpio; Dose-Response Relationship, Drug; Earth and Environmental Science; Ecotoxicology; Environment; Environmental Chemistry; Environmental Health; Environmental science; Enzymes; Exposure; Fish; Fish Proteins - genetics; Fish Proteins - metabolism; Fungicides; Fungicides, Industrial - toxicity; Mancozeb; Maneb - toxicity; Manganese; Manganese - metabolism; Neurotoxicity; NF-E2-Related Factor 2 - genetics; NF-E2-Related Factor 2 - metabolism; Oxidative stress; Reactive oxygen species; Research Article; Rodents; Superoxide dismutase; Toxicity; Waste Water Technology; Water Management; Water Pollutants, Chemical - toxicity; Water Pollution Control; Zineb - toxicity
• ISSN: 0944-1344; 1614-7499
• DOI: 10.1007/s11356-018-1724-9

Manganese (Mn)-containing dithiocarbamates such as Mancozeb (MZ) have been shown to induce oxidative stress-related toxicity in rodents and humans. However, little is known about the neurotoxic effects induced by MZ in fish. In this study, carp ( Cyprinus carpio ) were exposed to non-lethal waterborne concentrations of MZ, and oxidative stress parameters as well as metal accumulation in fish brains were evaluated. The experimental groups were as follows: control, MZ 5 mg/L, and MZ 10 mg/L. Fish were exposed for 7 days, and then brain was removed and prepared for subsequent analysis of antioxidant enzymes, reactive oxygen species (ROS), and expression of Nrf2 and phosphoNrf2. In parallel, manganese (Mn) levels were evaluated in blood and brain tissues. Mn levels were significantly increased in blood and brain of MZ-exposed carps. In addition, a concentration-dependent increase ( p