A new virus species causing a disease of Japanese holly fern (Cyrtomium falcatum)

An RNA virus has been found associated with diseases of Japanese holly fern (Cyrtomium falcatum) and leather leaf fern (Rumohra adiantiformis). Diseased Japanese holly fern plants show intense yellows, mosaic, necrosis, ring spots, and reduced growth. The virus was successfully graft-transmitted to...

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Bibliographic Details
Published in:Phytopathology 2008-06, Vol.98 (6), p.S162-S162
Main Authors: Valverde, R A, Sabanadzovic, S
Format: Article
Language:English
Subjects:
Cyrtomium falcatum
Groundnut rosette virus
Idaeovirus
Raspberry bushy dwarf virus
Rumohra adiantiformis
Umbravirus
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Summary:An RNA virus has been found associated with diseases of Japanese holly fern (Cyrtomium falcatum) and leather leaf fern (Rumohra adiantiformis). Diseased Japanese holly fern plants show intense yellows, mosaic, necrosis, ring spots, and reduced growth. The virus was successfully graft-transmitted to healthy Japanese holly ferns, with symptoms developing three to five weeks after inoculation. Double-stranded RNA analysis from infected tissues revealed two major dsRNAs considered to be the replicative forms of the viral genome. Viral dsRNAs 1 and 2 were gel purified and used as templates for molecular cloning and sequencing. The genome organization of this novel virus was similar to that of Raspberry bushy dwarf virus (RBDV) (genus Idaeovirus). Blast searches showed distant relationships with RBDV (RNA-1) and Groundnut rosette virus (genus Umbravirus) (RNA-2). The name of Holly fern mottle virus (HFMV) is proposed for this new virus species. Primers for RT-PCR detection of the virus have been developed and successfully used to test infected ferns. An increase in the number of HFMV-infected plants in various locations has been observed, suggesting that there is a natural vector for the virus. Therefore, research is being conducted to determine the means of natural spread of this virus.
ISSN:0031-949X