Genetic diversity of Enterobacter cloacae

Enterobacter bulb rot, caused by the bacterium Enterobacter cloacae, results in an storage onion bulb decay. This disease is characterized by watersoaking and a discoloration of the inner scales. Numerous bacterial isolates were obtained from onion bulbs in Washington during the 2006-2008 storage se...

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Bibliographic Details
Published in:Phytopathology 2009-06, Vol.99 (6), p.S101-S101
Main Authors: Pena, JE, Peever, T L, Schroeder, B K
Format: Article
Language:English
Subjects:
Allium cepa
Enterobacter
Enterobacter agglomerans
Enterobacter cloacae
Enterobacter sakazakii
Erwinia rhapontici
Pectobacterium
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Summary:Enterobacter bulb rot, caused by the bacterium Enterobacter cloacae, results in an storage onion bulb decay. This disease is characterized by watersoaking and a discoloration of the inner scales. Numerous bacterial isolates were obtained from onion bulbs in Washington during the 2006-2008 storage seasons that exhibited rot. They were identified as facultative anaerobes that were arginine dihydrolase positive, indole negative, and unable to degrade pectin, all characteristics typical of the genus Enterobacter. Currently, no species-specific probes or primers are available for these taxa and it is important to confirm the identity of the causal isolates. A multilocus phylogenetic analysis of these bacterial strains is being used to genetically characterize the putative E. cloacae isolates from onions and to clarify their evolutionary relationships with environmental and medical isolates of E. cloacae. Genomic DNA was isolated from representative isolates and portions of the housekeeping genes acnA, gapA, icdA, mdh, mtlD, pgi, and proA were PCR amplified and sequenced. Representative GenBank sequences from other plant-pathogenic facultative anaerobes such as Enterobacter sakazakii, Enterobacter agglomerans, Dickeya dadantii, Pectobacterium atrosepticum, P. carotovorum subsp. carotovorum, P. wasabiae, P. betavasculorum, and Erwinia rhapontici were also included in the analysis.
ISSN:0031-949X