Genetic and morphologic characterization of four putative cylindrospermopsin producing species of the cyanobacterial genera Anabaena and Aphanizomenon

Cylindrospermopsin (CYN) is a potent hepatotoxic alkaloid that has been detected in freshwater samples worldwide and is produced by a number of cyanobacterial species, mainly of the genera Cylindrospermopsis, Aphanizomenon and Anabaena. Cylindrospermopsis raciborskii is a morphologically distinctive...

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Published in:Journal of plankton research 2009-05, Vol.31 (5), p.465-480
Main Authors: Stüken, Anke, Campbell, Rebecca J., Quesada, Antonio, Sukenik, Assaf, Dadheech, Pawan K., Wiedner, Claudia
Format: Article
Language:English
Subjects:
Anabaena
Animal and plant ecology
Animal, plant and microbial ecology
Aphanizomenon
Biological and medical sciences
Cyanobacteria
Cylindrospermopsin
Cylindrospermopsis
Cylindrospermopsis raciborskii
Fresh water
Freshwater
Fundamental and applied biological sciences. Psychology
General aspects
Inland water environment
Phylogenetics
Phylogeny
rRNA 16S
Species
Strains
Synecology
Taxa
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Summary:Cylindrospermopsin (CYN) is a potent hepatotoxic alkaloid that has been detected in freshwater samples worldwide and is produced by a number of cyanobacterial species, mainly of the genera Cylindrospermopsis, Aphanizomenon and Anabaena. Cylindrospermopsis raciborskii is a morphologically distinctive species which forms a genetically well-defined cluster. In contrast, some species within Aphanizomenon and Anabaena are morphologically not clearly assignable to either genera and both genera are polyphyletic. In the Cylindrospermopsis cluster CYN producing and non-producing strains co-occur, but it is not known if CYN producing and non-producing strains are closely related in Anabaena and Aphanizomenon. Here we attempt to disentangle the phylogenetic relationships of four taxa of the genera Anabaena and Aphanizomenon, some of which are known as CYN producers. We have sequenced and phylogenetically analysed partial sequences of the 16S rRNA gene, cpcBA-IGS and rpoC1 from 31 cyanobacteria isolates of the genera Aphanizomenon and Anabaena and have documented morphotypic characteristics of new and recently isolated strains. Our results do not corroborate the separation of Aph. gracile and Aph. flos-aquae into separate species. In contrast, they support the distinction of Ana. bergii and Aph. ovalisporum into distinct taxa. Further, Ana. bergii is most likely not a CYN producing species.
ISSN:0142-7873
1464-3774
DOI:10.1093/plankt/fbp011