The toxicity study on marine low-temperature lysozyme

• Published in: Food and chemical toxicology 2008-02, Vol.46 (2), p.604-609
• Main Authors: Yan, Chun-ling, Ding, Bo-xiao, Lan, Xiao-ming, Guo, Shen-bo, Xie, Yan-ying, Wang, Chun-bo
• Format: Article
• Language: English
• Subjects: Acute toxicity; Animals; Anti-Infective Agents - chemistry; Anti-Infective Agents - metabolism; Anti-Infective Agents - toxicity; Biological and medical sciences; Body Weight - drug effects; Characterization; Cold Temperature; Dose-Response Relationship, Drug; Drug Interactions; Eating - drug effects; Female; Lethal Dose 50; Male; Marine; Marine low-temperature lysozyme; Medical sciences; Metals - pharmacology; Mice; Microbial Sensitivity Tests; Micrococcus; Micrococcus lysodleikticus; Muramidase - chemistry; Muramidase - metabolism; Muramidase - toxicity; Rat; Rats; Rats, Wistar; Subchronic toxicity; Toxicology
• ISSN: 0278-6915; 1873-6351
• DOI: 10.1016/j.fct.2007.09.001

Marine low-temperature lysozyme is purified from a marine bacterium. The lysozyme can keep high activity at low-temperature and has broad-spectrum antibiotic reaction. This study was undertaken to investigate the major characteristics, acute and subchronic toxicity of marine low-temperature lysozyme. The relative molecular weight of this lysozyme was determined as approximate 16 kD; its optimum pH value and temperature towards Micrococcus lysodleikticus were pH 6.5 and 35 °C, respectively. The lysozyme activity was slightly enhanced by Zn 2+ and Cu 2+ and slightly inhibited by Mn 2+ and Ag +. The lysozyme showed good compatibility to many common chemical agents such as EDTA (0.1%), KH 2PO 4 (1.0%), etc. In experiments on acute toxicity, the drug was injected through the tail vein of mice, and intoxication symptoms and date of death were recorded. The 50% lethal dose (LD 50) of Marine low-temperature lysozyme and 95%, 99% confidence interval (CI) was calculated. The subchronic study was designed to determine whether effects progressed with repeated Marine low-temperature lysozyme exposure. Wistar rats were tested by daily intragastric administration of Marine low-temperature lysozyme at the doses of 1.0; 0.5; 0.25 g/kg bw for 90 days. The LD 50 value of lysozyme was 4530 mg/kg bw; 90 days of Marine low-temperature lysozyme treatment at three doses, and there is no significant difference on blood biochemistry and organ index in drug treatment groups compared to saline treatment group. There is no affirmative pathologic change of all the observed organs in this study. The present results suggest that Marine low-temperature lysozyme can be safely used at the dose of experiment applied.