Predominance of Tetragenococcus halophilus as the cause of sugar thick juice degradation

The industrial storage of sugar thick juice was simulated on a laboratory scale. Terminal Restriction Fragment Length Polymorphism (T-RFLP) analysis and the application of Clone Libraries in parallel with classical microbiology were used to study the bacterial diversity and all revealed a dominance...

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Bibliographic Details
Published in:Food microbiology 2008-02, Vol.25 (2), p.413-421
Main Authors: Justé, Annelies, Lievens, Bart, Klingeberg, Michael, Michiels, Chris W., Marsh, Terence L., Willems, Kris A.
Format: Article
Language:English
Subjects:
beet pulp
beet sugar
Biological and medical sciences
brix
Carbohydrate Metabolism
Clone library
clones
Colony Count, Microbial
DNA libraries
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
Food industries
Food Microbiology
Food Preservation - methods
food storage
Fundamental and applied biological sciences. Psychology
Industrial Microbiology - methods
lactic acid
Lactic acid bacteria
Lactobacillus - classification
Lactobacillus - genetics
Lactobacillus - metabolism
microbial activity
molecular sequence data
Phylogeny
Polymorphism, Restriction Fragment Length
restriction fragment length polymorphism
RNA, Ribosomal, 16S - genetics
soluble solids
Species Specificity
Specific PCR
Sugar thick juice
sugarcane juice
Terminal restriction fragment length polymorphism
Tetragenococcus halophilus
viability
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Summary:The industrial storage of sugar thick juice was simulated on a laboratory scale. Terminal Restriction Fragment Length Polymorphism (T-RFLP) analysis and the application of Clone Libraries in parallel with classical microbiology were used to study the bacterial diversity and all revealed a dominance (>99%) of Tetragenococcus halophilus during storage. The degradation of thick juice correlated with the appearance of l-lactic acid and high concentrations of T. halophilus. In addition, pure cultures of T. halophilus induced degradation of sterile thick juice. A specific PCR was developed to detect T. halophilus and industrial thick juice samples from Belgium, Germany and France all contained T. halophilus, suggesting a consistent association of this organism with thick juice. T. halophilus has been known only as a halophile thus far, and this report is the first to show an association of this organism with a sugar-rich environment.
ISSN:0740-0020
1095-9998
DOI:10.1016/j.fm.2007.10.012