Effect of industrial trans-fatty acids-enriched diet on gut microbiota of C57BL/6 mice

Purpose Previous studies have shown that industrially originated trans -fatty acids ( i TFAs) are associated with several chronic diseases, but the underlying mechanisms remain unknown. Because gut microbiota play a critical role in human health, diet competent induced gut microbiota dysbiosis may c...

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Bibliographic Details
Published in:European journal of nutrition 2019-10, Vol.58 (7), p.2625-2638
Main Authors: Ge, Yueting, Liu, Wei, Tao, Haiteng, Zhang, Yu, Liu, Lina, Liu, Zhenhua, Qiu, Bin, Xu, Tongcheng
Format: Article
Language:English
Subjects:
Abundance
Animals
Bacteria
Butyric acid
Chemistry
Chemistry and Materials Science
Chronic illnesses
Diet
Diet - adverse effects
Digestive system
Dysbacteriosis
Fatty acids
Gas chromatography
Gastrointestinal Microbiome - drug effects
Gastrointestinal tract
Hydrogenation
Inflammation
Intestinal microflora
Male
Mice
Mice, Inbred C57BL
Microbiota
Nutrition
Original Contribution
Probiotics
rRNA 16S
Small intestine
Soybean Oil - administration & dosage
Soybeans
Trans Fatty Acids - administration & dosage
Trans Fatty Acids - adverse effects
Valeric acid
Vegetable oils
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Summary:Purpose Previous studies have shown that industrially originated trans -fatty acids ( i TFAs) are associated with several chronic diseases, but the underlying mechanisms remain unknown. Because gut microbiota play a critical role in human health, diet competent induced gut microbiota dysbiosis may contributing to disease pathogenesis. Therefore, the present study examined the impact of i TFA on gut microbiota, help understanding the underling mechanism of i TFA-associated chronic diseases. Methods Forty male 8-week-old mice were divided into 4 groups and randomly assigned to diets containing soybean oil (non- i TFA) or partially hydrogenated soybean oil ( i TFA). The intervention groups were: (1) low soybean oil (LS); (2) high soybean oil (HS); (3) low partially hydrogenated oil (LH) and (4) high partially hydrogenated oil (HH). The gut microbiota profiles were determined by 16S rRNA gene sequencing. Physiological parameters and the inflammatory status of the small intestine and other tissues were analyzed. Short-chain fatty acid levels in feces were measured using gas chromatography. Results The intake of i TFA increased the abundance of well-documented ‘harmful’ bacteria, such as Proteobacteria and Desulfovibrionaceae ( P  
ISSN:1436-6207
1436-6215
DOI:10.1007/s00394-018-1810-2