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Effect of industrial trans-fatty acids-enriched diet on gut microbiota of C57BL/6 mice
Purpose Previous studies have shown that industrially originated trans -fatty acids ( i TFAs) are associated with several chronic diseases, but the underlying mechanisms remain unknown. Because gut microbiota play a critical role in human health, diet competent induced gut microbiota dysbiosis may c...
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| Published in: | European journal of nutrition 2019-10, Vol.58 (7), p.2625-2638 |
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| container_end_page | 2638 |
| container_issue | 7 |
| container_start_page | 2625 |
| container_title | European journal of nutrition |
| container_volume | 58 |
| creator | Ge, Yueting Liu, Wei Tao, Haiteng Zhang, Yu Liu, Lina Liu, Zhenhua Qiu, Bin Xu, Tongcheng |
| description | Purpose
Previous studies have shown that industrially originated
trans
-fatty acids (
i
TFAs) are associated with several chronic diseases, but the underlying mechanisms remain unknown. Because gut microbiota play a critical role in human health, diet competent induced gut microbiota dysbiosis may contributing to disease pathogenesis. Therefore, the present study examined the impact of
i
TFA on gut microbiota, help understanding the underling mechanism of
i
TFA-associated chronic diseases.
Methods
Forty male 8-week-old mice were divided into 4 groups and randomly assigned to diets containing soybean oil (non-
i
TFA) or partially hydrogenated soybean oil (
i
TFA). The intervention groups were: (1) low soybean oil (LS); (2) high soybean oil (HS); (3) low partially hydrogenated oil (LH) and (4) high partially hydrogenated oil (HH). The gut microbiota profiles were determined by 16S rRNA gene sequencing. Physiological parameters and the inflammatory status of the small intestine and other tissues were analyzed. Short-chain fatty acid levels in feces were measured using gas chromatography.
Results
The intake of
i
TFA increased the abundance of well-documented ‘harmful’ bacteria, such as
Proteobacteria
and
Desulfovibrionaceae
(
P
|
| doi_str_mv | 10.1007/s00394-018-1810-2 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2089856989</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2089856989</sourcerecordid><originalsourceid>FETCH-LOGICAL-c438t-aaa79803b4ca7e626ec24c7690aa0483cde078a1f0521caec0c1d80366f088a63</originalsourceid><addsrcrecordid>eNp1kM1OAyEURonRWK0-gBsziRs32AvMMMxSm_qTNHGjbsktw1SadqYCs-jbS9NaExNXEDjfd-EQcsXgjgGUowAgqpwCU5QpBpQfkTOWC0klZ8XxYQ_lgJyHsAAALiQ7JQMBjEMh1Bn5mDSNNTHrmsy1dR-id7jMosc20AZj3GRoXB2obb0zn7bOamcT3WbzPmYrZ3w3c13EbX5clA_Tkdye2gty0uAy2Mv9OiTvj5O38TOdvj69jO-n1ORCRYqIZaVAzHKDpZVcWsNzU8oKECFXwtQWSoWsgYIzg9aAYXXipWxAKZRiSG53vWvfffU2RL1ywdjlElvb9UFzUJUqZKWqhN78QRdd79v0ukRVIHmRM5EotqPSz0LwttFr71boN5qB3krXO-k6Sddb6ZqnzPW-uZ-tbH1I_FhOAN8BIV21c-t_R__f-g2MgIpO</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2090625413</pqid></control><display><type>article</type><title>Effect of industrial trans-fatty acids-enriched diet on gut microbiota of C57BL/6 mice</title><source>Springer Nature</source><source>SPORTDiscus with Full Text</source><creator>Ge, Yueting ; Liu, Wei ; Tao, Haiteng ; Zhang, Yu ; Liu, Lina ; Liu, Zhenhua ; Qiu, Bin ; Xu, Tongcheng</creator><creatorcontrib>Ge, Yueting ; Liu, Wei ; Tao, Haiteng ; Zhang, Yu ; Liu, Lina ; Liu, Zhenhua ; Qiu, Bin ; Xu, Tongcheng</creatorcontrib><description>Purpose
Previous studies have shown that industrially originated
trans
-fatty acids (
i
TFAs) are associated with several chronic diseases, but the underlying mechanisms remain unknown. Because gut microbiota play a critical role in human health, diet competent induced gut microbiota dysbiosis may contributing to disease pathogenesis. Therefore, the present study examined the impact of
i
TFA on gut microbiota, help understanding the underling mechanism of
i
TFA-associated chronic diseases.
Methods
Forty male 8-week-old mice were divided into 4 groups and randomly assigned to diets containing soybean oil (non-
i
TFA) or partially hydrogenated soybean oil (
i
TFA). The intervention groups were: (1) low soybean oil (LS); (2) high soybean oil (HS); (3) low partially hydrogenated oil (LH) and (4) high partially hydrogenated oil (HH). The gut microbiota profiles were determined by 16S rRNA gene sequencing. Physiological parameters and the inflammatory status of the small intestine and other tissues were analyzed. Short-chain fatty acid levels in feces were measured using gas chromatography.
Results
The intake of
i
TFA increased the abundance of well-documented ‘harmful’ bacteria, such as
Proteobacteria
and
Desulfovibrionaceae
(
P
< 0.05), whereas it decreased relative abundance of ‘beneficial’ bacteria, such as
Bacteroidetes, Lachnospiraceae, Bacteroidales S24-7
(
P
< 0.05). Surprisingly, the intake of
i
TFA increased the abundance of the probiotic
Lactobacillaceae
(
P
< 0.05). Additionally, the intake of
i
TFA induced increase of inflammatory parameters, as well as a numerical decrease of fecal butyric acid and valeric acid.
Conclusions
This study, to our knowledge, is the first to demonstrate that the consumption of
i
TFA resulted in a significant dysbiosis of gut microbiota, which may contribute to the development of chronic diseases associated with
i
TFA.</description><identifier>ISSN: 1436-6207</identifier><identifier>EISSN: 1436-6215</identifier><identifier>DOI: 10.1007/s00394-018-1810-2</identifier><identifier>PMID: 30120538</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Abundance ; Animals ; Bacteria ; Butyric acid ; Chemistry ; Chemistry and Materials Science ; Chronic illnesses ; Diet ; Diet - adverse effects ; Digestive system ; Dysbacteriosis ; Fatty acids ; Gas chromatography ; Gastrointestinal Microbiome - drug effects ; Gastrointestinal tract ; Hydrogenation ; Inflammation ; Intestinal microflora ; Male ; Mice ; Mice, Inbred C57BL ; Microbiota ; Nutrition ; Original Contribution ; Probiotics ; rRNA 16S ; Small intestine ; Soybean Oil - administration & dosage ; Soybeans ; Trans Fatty Acids - administration & dosage ; Trans Fatty Acids - adverse effects ; Valeric acid ; Vegetable oils</subject><ispartof>European journal of nutrition, 2019-10, Vol.58 (7), p.2625-2638</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2018</rights><rights>European Journal of Nutrition is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-aaa79803b4ca7e626ec24c7690aa0483cde078a1f0521caec0c1d80366f088a63</citedby><cites>FETCH-LOGICAL-c438t-aaa79803b4ca7e626ec24c7690aa0483cde078a1f0521caec0c1d80366f088a63</cites><orcidid>0000-0003-0358-805X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30120538$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ge, Yueting</creatorcontrib><creatorcontrib>Liu, Wei</creatorcontrib><creatorcontrib>Tao, Haiteng</creatorcontrib><creatorcontrib>Zhang, Yu</creatorcontrib><creatorcontrib>Liu, Lina</creatorcontrib><creatorcontrib>Liu, Zhenhua</creatorcontrib><creatorcontrib>Qiu, Bin</creatorcontrib><creatorcontrib>Xu, Tongcheng</creatorcontrib><title>Effect of industrial trans-fatty acids-enriched diet on gut microbiota of C57BL/6 mice</title><title>European journal of nutrition</title><addtitle>Eur J Nutr</addtitle><addtitle>Eur J Nutr</addtitle><description>Purpose
Previous studies have shown that industrially originated
trans
-fatty acids (
i
TFAs) are associated with several chronic diseases, but the underlying mechanisms remain unknown. Because gut microbiota play a critical role in human health, diet competent induced gut microbiota dysbiosis may contributing to disease pathogenesis. Therefore, the present study examined the impact of
i
TFA on gut microbiota, help understanding the underling mechanism of
i
TFA-associated chronic diseases.
Methods
Forty male 8-week-old mice were divided into 4 groups and randomly assigned to diets containing soybean oil (non-
i
TFA) or partially hydrogenated soybean oil (
i
TFA). The intervention groups were: (1) low soybean oil (LS); (2) high soybean oil (HS); (3) low partially hydrogenated oil (LH) and (4) high partially hydrogenated oil (HH). The gut microbiota profiles were determined by 16S rRNA gene sequencing. Physiological parameters and the inflammatory status of the small intestine and other tissues were analyzed. Short-chain fatty acid levels in feces were measured using gas chromatography.
Results
The intake of
i
TFA increased the abundance of well-documented ‘harmful’ bacteria, such as
Proteobacteria
and
Desulfovibrionaceae
(
P
< 0.05), whereas it decreased relative abundance of ‘beneficial’ bacteria, such as
Bacteroidetes, Lachnospiraceae, Bacteroidales S24-7
(
P
< 0.05). Surprisingly, the intake of
i
TFA increased the abundance of the probiotic
Lactobacillaceae
(
P
< 0.05). Additionally, the intake of
i
TFA induced increase of inflammatory parameters, as well as a numerical decrease of fecal butyric acid and valeric acid.
Conclusions
This study, to our knowledge, is the first to demonstrate that the consumption of
i
TFA resulted in a significant dysbiosis of gut microbiota, which may contribute to the development of chronic diseases associated with
i
TFA.</description><subject>Abundance</subject><subject>Animals</subject><subject>Bacteria</subject><subject>Butyric acid</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chronic illnesses</subject><subject>Diet</subject><subject>Diet - adverse effects</subject><subject>Digestive system</subject><subject>Dysbacteriosis</subject><subject>Fatty acids</subject><subject>Gas chromatography</subject><subject>Gastrointestinal Microbiome - drug effects</subject><subject>Gastrointestinal tract</subject><subject>Hydrogenation</subject><subject>Inflammation</subject><subject>Intestinal microflora</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Microbiota</subject><subject>Nutrition</subject><subject>Original Contribution</subject><subject>Probiotics</subject><subject>rRNA 16S</subject><subject>Small intestine</subject><subject>Soybean Oil - administration & dosage</subject><subject>Soybeans</subject><subject>Trans Fatty Acids - administration & dosage</subject><subject>Trans Fatty Acids - adverse effects</subject><subject>Valeric acid</subject><subject>Vegetable oils</subject><issn>1436-6207</issn><issn>1436-6215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp1kM1OAyEURonRWK0-gBsziRs32AvMMMxSm_qTNHGjbsktw1SadqYCs-jbS9NaExNXEDjfd-EQcsXgjgGUowAgqpwCU5QpBpQfkTOWC0klZ8XxYQ_lgJyHsAAALiQ7JQMBjEMh1Bn5mDSNNTHrmsy1dR-id7jMosc20AZj3GRoXB2obb0zn7bOamcT3WbzPmYrZ3w3c13EbX5clA_Tkdye2gty0uAy2Mv9OiTvj5O38TOdvj69jO-n1ORCRYqIZaVAzHKDpZVcWsNzU8oKECFXwtQWSoWsgYIzg9aAYXXipWxAKZRiSG53vWvfffU2RL1ywdjlElvb9UFzUJUqZKWqhN78QRdd79v0ukRVIHmRM5EotqPSz0LwttFr71boN5qB3krXO-k6Sddb6ZqnzPW-uZ-tbH1I_FhOAN8BIV21c-t_R__f-g2MgIpO</recordid><startdate>20191001</startdate><enddate>20191001</enddate><creator>Ge, Yueting</creator><creator>Liu, Wei</creator><creator>Tao, Haiteng</creator><creator>Zhang, Yu</creator><creator>Liu, Lina</creator><creator>Liu, Zhenhua</creator><creator>Qiu, Bin</creator><creator>Xu, Tongcheng</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7RQ</scope><scope>7RV</scope><scope>7TS</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>M0R</scope><scope>M0S</scope><scope>M1P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-0358-805X</orcidid></search><sort><creationdate>20191001</creationdate><title>Effect of industrial trans-fatty acids-enriched diet on gut microbiota of C57BL/6 mice</title><author>Ge, Yueting ; Liu, Wei ; Tao, Haiteng ; Zhang, Yu ; Liu, Lina ; Liu, Zhenhua ; Qiu, Bin ; Xu, Tongcheng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-aaa79803b4ca7e626ec24c7690aa0483cde078a1f0521caec0c1d80366f088a63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Abundance</topic><topic>Animals</topic><topic>Bacteria</topic><topic>Butyric acid</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chronic illnesses</topic><topic>Diet</topic><topic>Diet - adverse effects</topic><topic>Digestive system</topic><topic>Dysbacteriosis</topic><topic>Fatty acids</topic><topic>Gas chromatography</topic><topic>Gastrointestinal Microbiome - drug effects</topic><topic>Gastrointestinal tract</topic><topic>Hydrogenation</topic><topic>Inflammation</topic><topic>Intestinal microflora</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Microbiota</topic><topic>Nutrition</topic><topic>Original Contribution</topic><topic>Probiotics</topic><topic>rRNA 16S</topic><topic>Small intestine</topic><topic>Soybean Oil - administration & dosage</topic><topic>Soybeans</topic><topic>Trans Fatty Acids - administration & dosage</topic><topic>Trans Fatty Acids - adverse effects</topic><topic>Valeric acid</topic><topic>Vegetable oils</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ge, Yueting</creatorcontrib><creatorcontrib>Liu, Wei</creatorcontrib><creatorcontrib>Tao, Haiteng</creatorcontrib><creatorcontrib>Zhang, Yu</creatorcontrib><creatorcontrib>Liu, Lina</creatorcontrib><creatorcontrib>Liu, Zhenhua</creatorcontrib><creatorcontrib>Qiu, Bin</creatorcontrib><creatorcontrib>Xu, Tongcheng</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>ProQuest Career and Technical Education</collection><collection>Nursing & Allied Health Database</collection><collection>Physical Education Index</collection><collection>ProQuest_Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>Consumer Health Database (Alumni Edition)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Consumer Health Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of nutrition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ge, Yueting</au><au>Liu, Wei</au><au>Tao, Haiteng</au><au>Zhang, Yu</au><au>Liu, Lina</au><au>Liu, Zhenhua</au><au>Qiu, Bin</au><au>Xu, Tongcheng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of industrial trans-fatty acids-enriched diet on gut microbiota of C57BL/6 mice</atitle><jtitle>European journal of nutrition</jtitle><stitle>Eur J Nutr</stitle><addtitle>Eur J Nutr</addtitle><date>2019-10-01</date><risdate>2019</risdate><volume>58</volume><issue>7</issue><spage>2625</spage><epage>2638</epage><pages>2625-2638</pages><issn>1436-6207</issn><eissn>1436-6215</eissn><abstract>Purpose
Previous studies have shown that industrially originated
trans
-fatty acids (
i
TFAs) are associated with several chronic diseases, but the underlying mechanisms remain unknown. Because gut microbiota play a critical role in human health, diet competent induced gut microbiota dysbiosis may contributing to disease pathogenesis. Therefore, the present study examined the impact of
i
TFA on gut microbiota, help understanding the underling mechanism of
i
TFA-associated chronic diseases.
Methods
Forty male 8-week-old mice were divided into 4 groups and randomly assigned to diets containing soybean oil (non-
i
TFA) or partially hydrogenated soybean oil (
i
TFA). The intervention groups were: (1) low soybean oil (LS); (2) high soybean oil (HS); (3) low partially hydrogenated oil (LH) and (4) high partially hydrogenated oil (HH). The gut microbiota profiles were determined by 16S rRNA gene sequencing. Physiological parameters and the inflammatory status of the small intestine and other tissues were analyzed. Short-chain fatty acid levels in feces were measured using gas chromatography.
Results
The intake of
i
TFA increased the abundance of well-documented ‘harmful’ bacteria, such as
Proteobacteria
and
Desulfovibrionaceae
(
P
< 0.05), whereas it decreased relative abundance of ‘beneficial’ bacteria, such as
Bacteroidetes, Lachnospiraceae, Bacteroidales S24-7
(
P
< 0.05). Surprisingly, the intake of
i
TFA increased the abundance of the probiotic
Lactobacillaceae
(
P
< 0.05). Additionally, the intake of
i
TFA induced increase of inflammatory parameters, as well as a numerical decrease of fecal butyric acid and valeric acid.
Conclusions
This study, to our knowledge, is the first to demonstrate that the consumption of
i
TFA resulted in a significant dysbiosis of gut microbiota, which may contribute to the development of chronic diseases associated with
i
TFA.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>30120538</pmid><doi>10.1007/s00394-018-1810-2</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0003-0358-805X</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1436-6207 |
| ispartof | European journal of nutrition, 2019-10, Vol.58 (7), p.2625-2638 |
| issn | 1436-6207 1436-6215 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2089856989 |
| source | Springer Nature; SPORTDiscus with Full Text |
| subjects | Abundance Animals Bacteria Butyric acid Chemistry Chemistry and Materials Science Chronic illnesses Diet Diet - adverse effects Digestive system Dysbacteriosis Fatty acids Gas chromatography Gastrointestinal Microbiome - drug effects Gastrointestinal tract Hydrogenation Inflammation Intestinal microflora Male Mice Mice, Inbred C57BL Microbiota Nutrition Original Contribution Probiotics rRNA 16S Small intestine Soybean Oil - administration & dosage Soybeans Trans Fatty Acids - administration & dosage Trans Fatty Acids - adverse effects Valeric acid Vegetable oils |
| title | Effect of industrial trans-fatty acids-enriched diet on gut microbiota of C57BL/6 mice |
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