Vairimorpha ephestiae is a synonym of Vairimorpha necatrix (Opisthosporidia: Microsporidia) based on multilocus sequence analysis

• Published in: European journal of protistology 2018-10, Vol.66, p.63-67
• Main Authors: Malysh, Julia M., Vorontsova, Yana L., Glupov, Viktor V., Tsarev, Alexander A., Tokarev, Yuri S.
• Format: Article
• Language: English
• Subjects: Microsporidia; Protein coding genes; Ribosomal DNA; Sequence similarity; Species identification
• ISSN: 0932-4739; 1618-0429
• DOI: 10.1016/j.ejop.2018.08.004

[Display omitted] •Vairimorpha ephestiae from Ephestia kühniella was propagated in Galleria mellonella.•Only one type of sporogony was observed and additional sporogony could not be induced.•Genotyping showed 98–100% sequence similarity to Vairimorpha necatrix.•Vairimorpha ephestiae is synonymized with Vairimorpha necatrix. An isolate of the microsporidium Vairimorpha ephestiae (originally isolated from Ephestia kühniella) from collection of Prof. J. Weiser was propagated in a laboratory culture of Galleria mellonella. Only disporoblastic sporogony was observed and formation of octospores, characteristic of the genus Vairimorpha, never occurred. A partial nucleotide sequence of the small subunit rRNA gene (1247 bp) for this microsporidium showed 100% identity to the homologous sequences of Vairimorpha (Nosema) necatrix (Genbank accession # U11051 and # DQ996241), a microsporidium with a broad host range within the Lepidoptera. Sequence similarity of protein-coding genes (RPB1, HSP70 and actin) between V. ephestiae and V. necatrix was about 98–100%. The level of genetic polymorphism in the RPB1 locus between these two species was essentially the same as between isolates of V. necatrix. It is therefore concluded that V. ephestiae is in fact an isolate of V. necatrix and the former species should be synonymized with the latter. Though described later, V. necatrix has prevailing usage and its precedence over V. ephestiae is proposed to conserve stability and avoid confusion.