Effects of ompA Deletion on Expression of Type 1 Fimbriae in Escherichia coli K1 Strain RS218 and on the Association of E. coli with Human Brain Microvascular Endothelial Cells

• Published in: Infection and Immunity 2006-10, Vol.74 (10), p.5609-5616
• Main Authors: Teng, Ching-Hao, Xie, Yi, Shin, Sooan, Di Cello, Francescopaolo, Paul-Satyaseela, Maneesh, Cai, Mian, Kim, Kwang Sik
• Format: Article
• Language: English
• Subjects: Bacterial Outer Membrane Proteins - genetics; Bacteriology; Biological and medical sciences; Brain - blood supply; Brain - microbiology; Capillaries - cytology; Capillaries - microbiology; Cells, Cultured; Endothelium, Vascular - microbiology; Escherichia coli; Escherichia coli - genetics; Escherichia coli - pathogenicity; Fimbria; Fimbriae, Bacterial - genetics; Fimbriae, Bacterial - metabolism; Fundamental and applied biological sciences. Psychology; Gene Deletion; Gene Expression Profiling; Humans; Microbiology; Miscellaneous; Molecular Pathogenesis; Multigene Family; Oligonucleotide Array Sequence Analysis; Promoter Regions, Genetic
• ISSN: 0019-9567; 1098-5522
• DOI: 10.1128/IAI.00321-06

We have previously shown that outer membrane protein A (OmpA) and type 1 fimbriae are the bacterial determinants involved in Escherichia coli K1 binding to human brain microvascular endothelial cells (HBMEC), which constitute the blood-brain barrier. In investigating the role of OmpA in E. coli K1 binding to HBMEC, we showed for the first time that ompA deletion decreased the expression of type 1 fimbriae in E. coli K1. Decreased expression of type 1 fimbriae in the ompA deletion mutant was largely the result of driving the fim promoter toward the type 1 fimbrial phase-OFF orientation. mRNA levels of fimB and fimE were found to be decreased with the OmpA mutant compared to the parent strain. Of interest, the ompA deletion further decreased the abilities of E. coli K1 to bind to and invade HBMEC under the conditions of fixing type 1 fimbria expression in the phase-ON or phase-OFF status. These findings suggest that the decreased ability of the OmpA mutant to interact with HBMEC is not entirely due to its decreased type 1 fimbrial expression and that OmpA and type 1 fimbriae facilitate the interaction of E. coli K1 with HBMEC at least in an additive manner.