Intranasal immunization with immunodominant epitope peptides derived from HpaA conjugated with CpG adjuvant protected mice against Helicobacter pylori infection

HpaA is considered to be an effective protective antigen for vaccination against Helicobacter pylori (H. pylori) infection. Oral immunization with HpaA significantly decreases bacterial colonization in H. pylori infected mice. In this study, we investigated whether subcutaneous or intranasal immuniz...

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Bibliographic Details
Published in:Vaccine 2018-10, Vol.36 (42), p.6301-6306
Main Authors: Yang, Wu-Chen, Sun, Hong-Wu, Sun, He-Qiang, Yuan, Han-Mei, Li, Bin, Li, Hai-Bo, Hu, Jian, Yang, Yun, Zou, Quan-Ming, Guo, Hong, Wu, Chao, Chen, Li
Format: Article
Language:English
Subjects:
Adjuvants
Adjuvants, Immunologic
Administration, Intranasal
Animals
Antibodies, Bacterial - immunology
Antigens
Cholera
Colonization
CpG islands
Epitopes
Female
Flow Cytometry
H. pylori
Helicobacter Infections - immunology
Helicobacter Infections - prevention & control
Helicobacter pylori
Helicobacter pylori - immunology
Helicobacter pylori - pathogenicity
HpaA
Immunization
Immunization - methods
Immunodominance
Immunodominant epitope
Immunodominant Epitopes - immunology
Infections
Investigations
Lymphocytes
Lymphocytes T
Mice
Mice, Inbred BALB C
Peptides
Peptides - administration & dosage
Peptides - immunology
Protective antigen
Spleen
T cell receptors
Vaccination
Vaccination - methods
Vaccines
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Summary:HpaA is considered to be an effective protective antigen for vaccination against Helicobacter pylori (H. pylori) infection. Oral immunization with HpaA significantly decreases bacterial colonization in H. pylori infected mice. In this study, we investigated whether subcutaneous or intranasal immunization with HpaA could protect against H. pylori infection. Mice immunized subcutaneously with HpaA in Complete Freund’s adjuvant, but not mice intranasally immunized with HpaA in CpG adjuvant acquired protection against H. pylori infection. However, intranasal immunization with immunodominant epitope peptides in CpG adjuvant protected mice against H. pylori infection, and immunodominant epitope peptides stimulated stronger Th1 responses and mediated more robust protection against H. pylori infection than subdominant ones. Our results suggest that the length of a candidate antigen is critical for particular vaccination routes, and that immunodominant epitope peptides are promising candidates for protection against H. pylori infection through nasal vaccination.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2018.09.007