A novel pH-responsive quaternary ammonium chitosan-liposome nanoparticles for periodontal treatment

The aim of this study was to evaluate the antibacterial activity and cytocompatibility of novel pH-activated nanoparticles (NPs) in vitro and in vivo. The NPs were synthesized from a quaternary ammonium chitosan, i.e., N,N,N-trimethyl chitosan, a liposome, and doxycycline (TMC-Lip-DOX NPs). The cyto...

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Bibliographic Details
Published in:International journal of biological macromolecules 2019-05, Vol.129, p.1113-1119
Main Authors: Hu, Fang, Zhou, Zhongzheng, Xu, Quanchen, Fan, Chun, Wang, Lei, Ren, Hao, Xu, Shuo, Ji, Qiuxia, Chen, Xiguang
Format: Article
Language:English
Subjects:
Ammonium Compounds - chemistry
Animal model
Animals
Anti-Bacterial Agents - chemistry
Anti-Bacterial Agents - pharmacology
Biofilms - drug effects
Chitosan - chemistry
Doxycycline
Doxycycline - chemistry
Doxycycline - pharmacology
Drug Carriers - chemistry
Histopathology
Hydrogen-Ion Concentration
Liposomes - chemistry
Materials Testing
Nanoparticles
Nanoparticles - chemistry
Optical Imaging
Periodontal Ligament - cytology
Periodontal Ligament - drug effects
Periodontal Ligament - microbiology
Periodontitis
Plaque biofilm
Porphyromonas gingivalis - drug effects
Prevotella intermedia - drug effects
Prevotella intermedia - physiology
Rats
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Summary:The aim of this study was to evaluate the antibacterial activity and cytocompatibility of novel pH-activated nanoparticles (NPs) in vitro and in vivo. The NPs were synthesized from a quaternary ammonium chitosan, i.e., N,N,N-trimethyl chitosan, a liposome, and doxycycline (TMC-Lip-DOX NPs). The cytocompatibility of the NPs was evaluated. The TMC-Lip-DOX NPs achieved superb inhibition of free mixed bacteria and biofilm formation. They also showed excellent biocompatibility with human periodontal ligament fibroblasts. Animal experiments showed that the NPs strongly inhibited biofilm formation and prevented alveolar bone absorption in vivo. All the results indicate that the TMC-Lip-DOX NPs have good potential for use in the treatment of periodontal and other inflammatory diseases. •The pH responding of Quaternary Ammonium Chitosan-liposome Nanoparticles could improve the efficacy of a given antibiotic to combat mixed bacterial biofilms.•Quaternary Ammonium Chitosan-liposome Nanoparticles have excellent Cytocompatibility.•The inhibition of gingival inflammation and alveolar bone loss by composite nanoparticles treatment is partly mediated by decreased bacterial plaque in root surface.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2018.09.057