Features of the formation of self-fertile euploid lines (2n = 42) by self-pollination of the 46-chromosome barley-wheat BC sub(1) hybrid Hordeum marinum subsp. gussoneanum Hudson (= H. geniculatum All.) (2n = 28) x Triticum aestivum L. (2n = 42)

• Published in: Russian journal of genetics 2006-12, Vol.42 (12), p.1422-1427
• Main Authors: Pershina, LA, Trubacheeva, N V, Rakovtseva, T S, Belova, LI, Devyatkina, E P, Kravtsova, LA
• Format: Article
• Language: English
• Subjects: Hordeum marinum; Hordeum vulgare; Triticum aestivum
• ISSN: 1022-7954
• DOI: 10.1134/S102279540612009X

We studied some features of the development of self-fertile 42-chromosome lines on the base of self-pollination progeny of 46-chromosome plants obtained by backcrossing of barley-wheat hybrids Hordeum marinum subsp. gussoneanum Hudson (= H. geniculatum All.) (2n = 28) x Triticum aestivum L. (2n = 42). The stabilization of karyotypes, resulting in 42-chromosome plants of the wheat type was generally completed by generation BC sub(1)F sub(10). The plants of all self-pollination progenies, including BC sub(1)F sub(10), showed some phenotypic traits characteristic of wild barley. Plants of BC sub(1)F sub(10) with the chromosome sets 2n = 42 and 2n = 42 + t were analyzed by RAPD with a set of 115 primers. Fragments of the wild barley genome were detected in RAPD patterns with 19 primers. Cross-hybridization confirmed that these fragments belonged to the wild barley genome. We raised four phenotypically different 42-chromosome lines from grains obtained from plants of generation BC sub(1)F sub(10), and these lines proved to be cytogenetically stable and self-fertile when grown in the field.