Detection of zearalenone and its metabolites in naturally contaminated follicular fluids by using LC/MS/MS and in vitro effects of zearalenone on oocyte maturation in cattle

Zearalenone (Zen) and its metabolites are estrogenic and may be important factors involved in reproductive disorders in domestic animals. We aimed to (1) simultaneously detect Zen and its metabolites in bovine follicular fluids (FFs) by liquid chromatography–tandem mass spectrometry and (2) examine...

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Published in:Reproductive toxicology (Elmsford, N.Y.) N.Y.), 2008-10, Vol.26 (2), p.164-169
Main Authors: Takagi, Mitsuhiro, Mukai, Shuhei, Kuriyagawa, Toshiyuki, Takagaki, Katsuhito, Uno, Seiichi, Kokushi, Emiko, Otoi, Takeshige, Budiyanto, Agung, Shirasuna, Koumei, Miyamoto, Akio, Kawamura, Osamu, Okamoto, Koji, Deguchi, Eisaburo
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cattle
Chromatography, Liquid
Embryology: invertebrates and vertebrates. Teratology
Embryonic Development - drug effects
Estrogens, Non-Steroidal - analysis
Estrogens, Non-Steroidal - metabolism
Estrogens, Non-Steroidal - toxicity
Female
Follicular fluid
Follicular Fluid - chemistry
Fundamental and applied biological sciences. Psychology
In Vitro Techniques
LC/MS/MS
Medical sciences
Oocyte maturation
Oocytes - drug effects
Plant poisons toxicology
Tandem Mass Spectrometry
Teratology. Teratogens
Toxicology
Zearalenone
Zearalenone - analysis
Zearalenone - metabolism
Zearalenone - toxicity
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Summary:Zearalenone (Zen) and its metabolites are estrogenic and may be important factors involved in reproductive disorders in domestic animals. We aimed to (1) simultaneously detect Zen and its metabolites in bovine follicular fluids (FFs) by liquid chromatography–tandem mass spectrometry and (2) examine the in vitro effects of Zen on bovine oocytes. Zen and its metabolites were detected in 6 of 32 normal follicles and 7 of 20 cystic follicles. Bovine oocytes were cultured in a maturation media containing various Zen concentrations (0 [control], 1, 10, 100, and 1000 μg/L), fertilized, and cultured further. Maturation rates decreased dose-dependently. Further, maturation of 62 (50%) of 124 oocytes examined in the 1000-μg/L group was arrested in metaphase I, without affecting the fertilization rate. Blastocyst-formation rates did not significantly differ among the groups. Zen and its metabolites were detectable in bovine FFs. High Zen concentration may adversely affect meiotic competence but not the fertilization and development rates.
ISSN:0890-6238
1873-1708
DOI:10.1016/j.reprotox.2008.08.006