A case of laboratory cross-contamination of Mycobacterium tuberculosis identified using comparative genomics

BACKGROUND: Two false-positive tuberculosis (TB) cases in Yamagata Prefecture, Japan, 2016.OBJECTIVE: To report the effectiveness of comparative genomics of Mycobacterium tuberculosis for identification of cross-contamination cases.DESIGN: Case report of laboratory cross-contamination.RESULTS: Begin...

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Bibliographic Details
Published in:The international journal of tuberculosis and lung disease 2018-10, Vol.22 (10), p.1239-1242
Main Authors: Seto, J., Wada, T., Suzuki, Y., Ikeda, T., Araki, K., Umetsu, Y., Ishikawa, H., Mizuta, K., Ahiko, T.
Format: Article
Language:English
Subjects:
Acid-Fast Bacilli Smear Test
Contamination
Epidemiology
False-Positive
Genomics
Genotypes
Laboratories
Mycobacterium tuberculosis
Side Effects
Strains (organisms)
Tuberculosis
Unnecessary Treatment
Vntr Typing
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Summary:BACKGROUND: Two false-positive tuberculosis (TB) cases in Yamagata Prefecture, Japan, 2016.OBJECTIVE: To report the effectiveness of comparative genomics of Mycobacterium tuberculosis for identification of cross-contamination cases.DESIGN: Case report of laboratory cross-contamination.RESULTS: Beginning with detection of an identical genotype in two M. tuberculosis strains using variable number of tandem repeat typing, we suspected M. tuberculosis cross-contamination of specimens collected in a mycobacteriology laboratory based on epidemiological investigations. This suspicion was confirmed using comparative genomics of the two M. tuberculosis strains and a strain from an epidemiologically unrelated specimen from the same batch as the two strains in the mycobacteriology laboratory. All strains had an identical genomic sequence with no single nucleotide variants.CONCLUSION: Comparative genomics, which offers the highest discrimination power, is a potent tool for identifying laboratory cross-contamination using epidemiological investigations.
ISSN:1027-3719
1815-7920
DOI:10.5588/ijtld.18.0237