Distribution of virulence factors, determinants of antibiotic resistance and molecular fingerprinting of Salmonella species isolated from cattle and beef samples: suggestive evidence of animal-to-meat contamination

• Published in: Environmental science and pollution research international 2018-11, Vol.25 (32), p.32694-32708
• Main Authors: Dlamini, Beauty Sicelo, Montso, Peter Kotsoana, Kumar, Ajay, Ateba, Collins Njie
• Format: Article
• Language: English
• Subjects: Animals; Anti-Bacterial Agents; Antibiotic resistance; Antibiotics; Aquatic Pollution; Atmospheric Protection/Air Quality Control/Air Pollution; Beef; Beef cattle; Biological Assay; Bovidae; Cattle; Chemical fingerprinting; Cluster Analysis; Determinants; Drug Resistance, Microbial; Earth and Environmental Science; Ecotoxicology; Environment; Environmental Chemistry; Environmental Health; Environmental science; Epidemiology; Feces; Food contamination; Food Microbiology; Fragmentation; Fragments; Gene polymorphism; Genetic fingerprinting; Hypersensitivity; Meat; Meat - microbiology; Ownership; Phenotype; Phenotypes; Polymerase Chain Reaction; Polymorphism; Polymorphism, Restriction Fragment Length; Red Meat; Research Article; Resistance factors; Restriction fragment length polymorphism; RNA, Ribosomal, 16S; rRNA 16S; Salmonella; Salmonella - growth & development; Salmonella - isolation & purification; Typing; Virulence; Virulence Factors; Waste Water Technology; Water Management; Water Pollution Control
• ISSN: 0944-1344; 1614-7499
• DOI: 10.1007/s11356-018-3231-4

In this study, three hundred presumptive Salmonella strains isolated from cattle faeces and raw beef samples were subjected to both preliminary and confirmatory tests specific for Salmonella . PCR assays revealed that 100%, 20% and 26.7% of the isolates were positive for 16S rRNA, fli C and flj B gene fragments, respectively. Large proportions (62.4 to 94.3%) of these isolates were multiple antibiotic resistant (MAR) strains that were resistant to three or more antibiotics belonging to different classes. MAR phenotypes Ab1, Ab2, Ab3, Ab7, Ab8, Ab9, Ab26 and Ab27 were dominant among the isolates. Cluster analysis of antibiotic inhibition zone diameter data revealed two major clusters (clusters 1 and 2), and each cluster contained two sub-clusters (1A, 1B, 2A and 2B). PCR data revealed that 27.1% and 30.7% of the isolates possessed the spv C and inv A virulent genes, respectively. There was a significant correlation between the possession of MAR phenotypes and virulent gene determinants. Analysis of restriction fragment length polymorphism (RFLP) of 16S rRNA gene fragments using Eco RI and Hae III showed that large proportions of isolates from beef and cattle faeces produced similar genetic fingerprints. From these results, it is suggested that Salmonella species in cattle are transmitted to beef and, therefore, the consumption of undercooked beef could pose severe health complications on consumers. These findings provide baseline data that could be of great epidemiological importance and, thus, the need to utilise more sensitive typing tools in determining the genetic relatedness of isolates from different sources.