Gene Discovery in Gelsemium Highlights Conserved Gene Clusters in Monoterpene Indole Alkaloid Biosynthesis

Genome mining is a routine technique in microbes for discovering biosynthetic pathways. In plants, however, genomic information is not commonly used to identify novel biosynthesis genes. Here, we present the genome of the medicinal plant and oxindole monoterpene indole alkaloid (MIA) producer Gelsem...

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Published in:Chembiochem : a European journal of chemical biology 2019-01, Vol.20 (1), p.83-87
Main Authors: Franke, Jakob, Kim, Jeongwoon, Hamilton, John P., Zhao, Dongyan, Pham, Gina M., Wiegert‐Rininger, Krystle, Crisovan, Emily, Newton, Linsey, Vaillancourt, Brieanne, Tatsis, Evangelos, Buell, C. Robin, O'Connor, Sarah E.
Format: Article
Language:English
Subjects:
Biosynthesis
Catharanthus - genetics
Gelsemium
Gelsemium - genetics
Gelsemium sempervirens
Gene clusters
Genes, Plant
Genetic Association Studies
Genome
Genomes
Herbal medicine
Indole Alkaloids - metabolism
Indoles
Medicinal plants
monoterpene indole alkaloid
Monoterpenes - metabolism
Multigene Family
Plant Roots - genetics
Plants (botany)
strictosidine
Substrates
synteny
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Summary:Genome mining is a routine technique in microbes for discovering biosynthetic pathways. In plants, however, genomic information is not commonly used to identify novel biosynthesis genes. Here, we present the genome of the medicinal plant and oxindole monoterpene indole alkaloid (MIA) producer Gelsemium sempervirens (Gelsemiaceae). A gene cluster from Catharanthus roseus, which is utilized at least six enzymatic steps downstream from the last common intermediate shared between the two plant alkaloid types, is found in G. sempervirens, although the corresponding enzymes act on entirely different substrates. This study provides insights into the common genomic context of MIA pathways and is an important milestone in the further elucidation of the Gelsemium oxindole alkaloid pathway. The genome of the oxindole monoterpene indole alkaloid producer G. sempervirens is reported. Notably, a gene cluster from the distantly related plant C. roseus is found in G. sempervirens. However, though the genomic organization of the cluster is conserved, the corresponding enzymes act on entirely different substrates.
ISSN:1439-4227
1439-7633
DOI:10.1002/cbic.201800592