Characterization of Capsicum annuum Recombinant a- and b-Tubulin

There are several conditions which might modulate polymerization to produce polymers having normal lattice structure. In the absence of 1mM MgCl sub(2) the assembly was reduced by 36% in Capsicum annuum tubulin (CAnm tubulin). There was no significant difference in the final assembly formation in th...

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Bibliographic Details
Published in:Applied biochemistry and biotechnology 2010-01, Vol.160 (1), p.122-128
Main Authors: Koo, Bon-Sung, Jang, Myung-Hyun, Park, Haechul, Kalme, Satish, Park, Hey-Yeon, Han, Jin-Wook, Yeo, Yun-Soo, Yoon, Sang-Hong, Kim, Soo-Jin, Lee, Chang-Muk, Yoon, Moon-Young
Format: Article
Language:English
Subjects:
Capsicum annuum
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Summary:There are several conditions which might modulate polymerization to produce polymers having normal lattice structure. In the absence of 1mM MgCl sub(2) the assembly was reduced by 36% in Capsicum annuum tubulin (CAnm tubulin). There was no significant difference in the final assembly formation in the presence of 5% to 10% glycerol. However, nucleation rate was slow and apparent study state was achieved lately in the presence of 10% glycerol. Taxol at 100kM concentration increased 23% tubulin assembly. One millimolar CaCl sub(2), .1% dimethyl sulfoxide (DMSO) and physiologically low temperature reduced CAnm tubulin assembly. A value of 0.089mg/ml was obtained as critical concentration for polymerization. Benomyl significantly reduced the number of cysteine residues accessible to 5,5'-dithiobis-(2-nitrobenzoic acid); there were 4.77c0.21 and 3.49c0.35 residues accessible per tubulin dimer in the presence of 50 and 100kM benomyl respectively.
ISSN:0273-2289
1559-0291
DOI:10.1007/s12010-008-8489-4