Four-Dimensional Imaging of Soft Tissue and Implanted Biomaterial Mechanics: A Barbed Suture Case Study for Tendon Repair

• Published in: ACS applied materials & interfaces 2018-11, Vol.10 (45), p.38681-38691
• Main Authors: Rawson, Shelley D, Shearer, Tom, Lowe, Tristan, O’Brien, Marie, Wong, Jason K. F, Margetts, Lee, Cartmell, Sarah H
• Format: Article
• Language: English
• Subjects: Animals; Biocompatible Materials - chemistry; Hand Injuries - surgery; Humans; Sutures; Swine; Tendon Injuries - diagnostic imaging; Tendon Injuries - surgery; Tendons - diagnostic imaging; Tendons - surgery; Tensile Strength; X-Ray Microtomography - methods
• ISSN: 1944-8244; 1944-8252
• DOI: 10.1021/acsami.8b09700

Timely, recent developments in X-ray microcomputed tomography (XμCT) imaging such as increased resolution and improved sample preparation enable nondestructive time-lapse imaging of polymeric biomaterials when implanted in soft tissue, which we demonstrate herein. Imaging the full three-dimensional (3D) structure of an implanted biomaterial provides new opportunities to assess the micromechanics of the interface between the implant and tissues and how this changes over time as force is applied in load-bearing musculoskeletal applications. In this paper, we present a case study demonstrating in situ XμCT and finite element analysis, using a dynamically loaded barbed suture repair for its novel use in tendon tissue. The aim of this study was to identify the distribution of stress in the suture and tendon as load is applied. The data gained demonstrate a clear 3D visualization of microscale features in both the tissue and implant in wet conditions. XμCT imaging has revealed, for the first time, pores around the suture, preventing full engagement of all the barbs with the tendon tissue. Subsequent finite element analysis reveals the localized stress and strain, which are not evenly distributed along the suture, or throughout the tissue. This case study demonstrates for the first time a powerful in situ mechanical imaging tool, which could be readily adapted by other laboratories to interrogate and optimize the interface between the implanted biomaterials and the soft tissue.