Intracellular and extracellular effector activity of mouse neutrophils in response to cutaneous and visceral Leishmania parasites

[Display omitted] •Neutrophils uptake Leishmania promastigotes and tolerate amastigote differentiation.•During early infection, parasites do not seem to signal through neutrophil PRRs.•Visceral and cutaneous species of Leishmania activate neutrophil oxidative burst.•The extracellular activity of neu...

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Bibliographic Details
Published in:Cellular immunology 2019-01, Vol.335, p.76-84
Main Authors: Valério-Bolas, Ana, Pereira, Maria, Alexandre-Pires, Graça, Santos-Mateus, David, Rodrigues, Armanda, Rafael-Fernandes, Mariana, Gabriel, Aurea, Passero, Felipe, Santos-Gomes, Gabriela
Format: Article
Language:English
Subjects:
Degranulation
Innate immunity
Leishmania spp
Mouse neutrophils
Neutrophil extracellular traps
Oxidative burst
Pattern recognition receptors
Phagocytosis
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Summary:[Display omitted] •Neutrophils uptake Leishmania promastigotes and tolerate amastigote differentiation.•During early infection, parasites do not seem to signal through neutrophil PRRs.•Visceral and cutaneous species of Leishmania activate neutrophil oxidative burst.•The extracellular activity of neutrophil enzymes is dependent on parasite species.•Neutrophils emit NETs against cutaneous and visceral species of Leishmania. Neutrophils are short-lived phagocytic cells equipped with several receptors for pathogen recognition and phagocytosis and have intracellular and extracellular effector mechanisms that can inactivate pathogens. Leishmaniases are diseases caused by different species of Leishmania that mainly afflicts poorer populations of tropical and subtropical regions and immunocompromised individuals. Thus, the present study aims to investigate the effector response of murine neutrophils to species of Leishmania causing American cutaneous leishmaniasis and zoonotic visceral leishmaniasis by evaluating pattern recognition receptors (PRR) and intracellular and extracellular effector microbicide activity. When exposed to Leishmania parasites, mouse neutrophils produced superoxide, released enzymes in the extracellular space and generated neutrophil extracellular traps, although PRR gene expression is negatively regulated. L. infantum, L. guyanensis, and L. shawi inhibited enzymatic activity, whereas L. amazonensis reduced the emission of extracellular structures. These findings indicate that although neutrophils trigger several microbicide mechanisms, Leishmania parasites can manipulate extracellular effector mechanisms. The present study also provides evidence that neutrophils can internalize parasites by coiling phagocytosis.
ISSN:0008-8749
1090-2163
DOI:10.1016/j.cellimm.2018.11.003