The Fine Art of Destruction: A Guide to In‐Depth Glycoproteomic Analyses—Exploiting the Diagnostic Potential of Fragment Ions

The unambiguous mass spectrometric identification and characterization of glycopeptides is crucial to elucidate the micro‐ and macroheterogeneity of glycoproteins. Here, combining lower and stepped collisional energy fragmentation for the in‐depth and site‐specific analysis of N‐ and O‐glycopeptides...

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Bibliographic Details
Published in:Proteomics (Weinheim) 2018-12, Vol.18 (24), p.e1800282-n/a
Main Authors: Hoffmann, Marcus, Pioch, Markus, Pralow, Alexander, Hennig, René, Kottler, Robert, Reichl, Udo, Rapp, Erdmann
Format: Article
Language:English
Subjects:
Animals
Cattle
fibrinogen
Fibrinogen - metabolism
Glycopeptides - analysis
Glycoproteins - analysis
glycoproteomics
Glycosylation
higher energy collision dissociation
Humans
hydrophilic interaction liquid chromatography
IgG
Immunoglobulin G - metabolism
Lactoferrin - metabolism
lactotransferrin
mass spectrometry
N‐glycosylation
oxonium ions
O‐glycosylation
Polysaccharides - metabolism
proteinase K
ribonuclease B
Ribonucleases - metabolism
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