Distribution of histone deacetylases 1-11 in the rat brain

Although protein phosphorylation has been characterized more extensively, modulation of the acetylation state of signaling molecules is now being recognized as a key means of signal transduction. The enzymes responsible for mediating these changes include histone acetyl transferases and histone deac...

Full description

Saved in:
Bibliographic Details
Published in:Journal of molecular neuroscience 2007, Vol.31 (1), p.47-58
Main Authors: Broide, Ron S, Redwine, Jeff M, Aftahi, Najla, Young, Warren, Bloom, Floyd E, Winrow, Christopher J
Format: Article
Language:English
Subjects:
Animals
Brain - cytology
Brain - enzymology
Gene expression
Gene Expression Profiling
Histone Deacetylases - genetics
Histone Deacetylases - metabolism
In Situ Hybridization
Isoenzymes - genetics
Isoenzymes - metabolism
Male
Neurons - enzymology
Oligodendroglia - enzymology
Proteins
Rats
Signal transduction
Tissue Distribution
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Although protein phosphorylation has been characterized more extensively, modulation of the acetylation state of signaling molecules is now being recognized as a key means of signal transduction. The enzymes responsible for mediating these changes include histone acetyl transferases and histone deacetylases (HDACs). Members of the HDAC family of enzymes have been identified as potential therapeutic targets for diseases ranging from cancer to ischemia and neurodegeneration. We initiated a project to conduct comprehensive gene expression mapping of the 11 HDAC isoforms (HDAC1-11) (classes I, II, and IV) throughout the rat brain using high-resolution in situ hybridization (ISH) and imaging technology. Internal and external data bases were employed to identify the appropriate rat sequence information for probe selection. In addition, immunohistochemistry was performed on these samples to separately examine HDAC expression in neurons, astrocytes, oligodendrocytes, and endothelial cells in the CNS. This double-labeling approach enabled the identification of specific cell types in which the individual HDACs were expressed. The signals obtained by ISH were compared to radiolabeled standards and thereby enabled semiquantitative analysis of individual HDAC isoforms and defined relative levels of gene expression in >50 brain regions. This project produced an extensive atlas of 11 HDAC isoforms throughout the rat brain, including cell type localization, providing a valuable resource for examining the roles of specific HDACs in the brain and the development of future modulators of HDAC activity.
ISSN:0895-8696
1559-1166
DOI:10.1007/bf02686117