Biotransformation of carbon tetrachloride by various acetate- and nitrate-limited denitrifying consortia

Fed-batch experiments were performed to determine the carbon tetrachloride (CT)-degrading ability of three denitrifying consortia cultured from sites not contaminated with CT. A mathematical model was used to quantify the rates of CT transformation by the consortia under both acetate-limiting and ni...

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Bibliographic Details
Published in:Biotechnology and bioengineering 1999-08, Vol.64 (3), p.342-348
Main Authors: Sherwood, J.L, Petersen, J.N, Skeen, R.S
Format: Article
Language:English
Subjects:
biodegradation
Biodegradation of pollutants
Biological and medical sciences
Bioremediation
Biotechnology
Carbon tetrachloride
consortia
Denitrification
Environment and pollution
Fundamental and applied biological sciences. Psychology
Industrial applications and implications. Economical aspects
Mathematical models
Rate constants
Soil pollution control
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Summary:Fed-batch experiments were performed to determine the carbon tetrachloride (CT)-degrading ability of three denitrifying consortia cultured from sites not contaminated with CT. A mathematical model was used to quantify the rates of CT transformation by the consortia under both acetate-limiting and nitrate-limiting conditions. A rate constant for CT transformation on a cellular protein basis and the fraction of degraded CT transformed to chloroform (CF) were determined for each consortium by optimizing the model to fit the experimental data. The parameters for these experiments were statistically compared to those obtained for previous experiments with a denitrifying consortium cultured from an aquifer soil sample from the US Department of Energy Hanford site in southeastern Washington state. Results of F-test analysis indicated the rate of CT transformation and the production of CF both were functions of the limiting nutrient. Under nitrate-limited conditions, the rate constant for CT transformation for all four consortia was about 30 L/gmol/min and approximately 50% of the CT transformed was converted to CF. When acetate was the limiting nutrient, the rate constant for CT transformation was approximately 8 L/gmol/min and the CF yield decreased to about 25%. These results imply the ability to degrade CT may be inherent to some denitrifying organisms, regardless of previous exposure to CT.
ISSN:0006-3592
1097-0290
DOI:10.1002/(SICI)1097-0290(19990805)64:3<342::AID-BIT10>3.0.CO;2-4