Cyclic mechanical strain alters the expression of procoagulant activity by rat osteosarcoma cells cultured on titanium substrates

Rat osteosarcoma cells (ROS) were cultured on titanium (Ti6Al4V), subjected to cyclic strain in a four-point bending apparatus, and subsequently assayed for surface tissue factor (TF) activity. TF, a transmembrane glycoprotein, plays a critical role in the initiation of blood coagulation at sites of...

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Bibliographic Details
Main Authors: Bledsoe, J.G., Slack, S.M.
Format: Conference Proceeding
Language:English
Subjects:
Blood
Bone
Bones
Calcium
Capacitive sensors
Cell culture
Cell membranes
Cells (biology)
Coagulation
Injuries
Oncology
Proteins
Strain
Strain measurement
Surface treatment
Titanium
Titanium alloys
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Summary:Rat osteosarcoma cells (ROS) were cultured on titanium (Ti6Al4V), subjected to cyclic strain in a four-point bending apparatus, and subsequently assayed for surface tissue factor (TF) activity. TF, a transmembrane glycoprotein, plays a critical role in the initiation of blood coagulation at sites of vascular injury and also participates in the inflammatory response. Thus, surface TF activity is indicative of the procoagulant properties of cells and those cells' ability to regulate the inflammatory response. In this study, the authors have shown that surface TF activity in ROS decreases with increasing stimulation time. Surface TF activity also depended on the form of applied strain. Strains were varied between +0.2% and -0.2% with maximum surface TF activity occurring at /spl plusmn/0.1%.
ISSN:1094-687X
0589-1019
1558-4615
DOI:10.1109/IEMBS.1999.803933