Cotransport of biochar and Shewanella oneidensis MR-1 in saturated porous media: Impacts of electrostatic interaction, extracellular electron transfer and microbial taxis

• Published in: The Science of the total environment 2019-03, Vol.658, p.95-104
• Main Authors: Liu, Lecheng, Liu, Guangfei, Zhou, Jiti, Wang, Jing, Jin, Ruofei
• Format: Article
• Language: English
• Subjects: Biochar; Cotransport; EET; Redox state; Shewanella; Taxis
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2018.12.031

Biochar widely applied to soil can influence microbial community composition and participate in extracellular electron transfer (EET). However, little is known about the cotransport behaviors of bacteria and biochar in aquifer and soil-water environments, which can affect the fate and application performance of biochar. In this study, we found that in comparison to their individual transport behaviors, the mobilities of cotransporting Shewanella oneidensis MR-1 and biochar colloid (BC) were significantly inhibited. The decreasing colloidal mobilities at higher ionic strengths signified the importance of electrostatic interaction between cell and BC in cotransport. Moreover, the less suppressed cotransport of BC and mutants defective of EET and the elevated inhibition effects on cotransport by adding exogenous electron donor suggested the importance of EET. Difference in cotransport behavior was also observed with BC having different redox states. Compared with oxidized BC, reduced BC with higher hydrophobicity led to easier aggregation with cell and higher retention in column. More importantly, MR-1 exhibited EET-dependent taxis towards biochar, which also contributed to the enhanced heteroaggregation and decreased mobilities of cell and biochar. Our results highlight that metabolic activities of microbes towards abiotic colloids cannot be neglected when assessing their transport behaviors, especially in subsurface environments abounded with redox-active inorganic particles and microbes performing extracellular respiration. [Display omitted] •Heteroaggregation of biochar and cell retards their cotransport.•Mobilities of biochar and cell decrease with increasing ionic strength.•Reduction increases biochar's hydrophobicity and inhibits cotransport.•EET-dependent taxis of cell towards biochar inhibits cotransport.