Transcriptome analysis of the brain of the Chinese mitten crab, Eriocheir sinensis, for neuropeptide abundance profiles during ovarian development

•The identification of neuropeptides and receptors from E. sinensis brain.•The differential abundance of neuropeptides during ovarian maturation.•Many neuropeptides have been shown to be involved in reproduction. Neuropeptides, important messenger molecules, regulate various physiological processes,...

Full description

Saved in:
Bibliographic Details
Published in:Animal reproduction science 2019-02, Vol.201, p.63-70
Main Authors: Liu, Xue, Ma, KeYi, Liu, ZhiQiang, Feng, JianBin, Ye, BaoQing, Qiu, GaoFeng
Format: Article
Language:English
Subjects:
Animals
Brachyura - metabolism
Brain - metabolism
Female
Neuropeptides
Neuropeptides - metabolism
Ovarian development
Ovary - physiology
Polymorphism, Single Nucleotide
RNA, Messenger - genetics
RNA, Messenger - metabolism
Transcriptome
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:•The identification of neuropeptides and receptors from E. sinensis brain.•The differential abundance of neuropeptides during ovarian maturation.•Many neuropeptides have been shown to be involved in reproduction. Neuropeptides, important messenger molecules, regulate various physiological processes, such as growth, development, and reproduction. In the present study, cDNA libraries from brains of E. sinensis were constructed and sequenced using the Illumina technique for transcript analysis and neuropeptides discovery. There were 233,887 transcripts assembled for 194,286 unigenes. According to the annotations of NCBI non-redundant protein (NR) database, 2487 (11.31%) unigenes were annotated successfully. In total, 1273 transcripts were assigned to the “signal transduction mechanisms” category using KOG analysis. The results of KEGG indicate signal transduction and translation pathways were the dominant and enriched signal pathways. Additionally, results indicated C2H2 was the main transcription factor (TF) family. Analysis of the assembled transcripts indicated there were 22 neuropeptide transcripts, such as allatostatin, crustacean female sex hormone, crustacean hyperglycemic hormone, diuretic hormone 31, and eclosion hormone. The detection of these neuropeptides provide for a basic understanding for future study of functions in development, reproduction, and sexual maturation in crustaceans.
ISSN:0378-4320
1873-2232
DOI:10.1016/j.anireprosci.2018.12.010