Histological, immunohistochemical, and genomic evaluation of excisional and diabetic wounds treated with human Wharton’s jelly stem cells with and without a nanocarrier

We showed in previous studies that human umbilical cord Wharton’s jelly stem cells (hWJSCs) improved the healing rates of excisional and diabetic wounds in the mouse model. As an extension of those studies, we report here the more detailed quantitative histological, immunohistochemical, and genomic...

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Bibliographic Details
Published in:Journal of cellular biochemistry 2019-07, Vol.120 (7), p.11222-11240
Main Authors: Raj, Vaishnevi, Claudine, Stephanie, Subramanian, Arjunan, Tam, Kimberley, Biswas, Arijit, Bongso, Ariff, Fong, Chui‐Yee
Format: Article
Language:English
Subjects:
Aloe
Angiogenesis
Biosynthesis
Cell proliferation
Collagen
Conditioning
Dermis
Diabetes
Diabetes mellitus
diabetic wounds
Epidermis
Evaluation
excisional wounds
Extracellular matrix
Gene expression
Granulation
histology and immunohistochemistry
human Wharton’s jelly stem cells
Immunohistochemistry
Immunomodulation
nanoscaffold
Paracrine signalling
Polycaprolactone
Polymerase chain reaction
Secretions
Stem cell transplantation
Stem cells
Topical application
Umbilical cord
Wound healing
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Summary:We showed in previous studies that human umbilical cord Wharton’s jelly stem cells (hWJSCs) improved the healing rates of excisional and diabetic wounds in the mouse model. As an extension of those studies, we report here the more detailed quantitative histological, immunohistochemical, and genomic evaluation of biopsies from those excisional and diabetic wounds in an attempt to understand the mechanisms of the enhanced wound healing aided by hWJSCs. Bright‐field microscopic observations and ImageJ software analysis on histological sections of the excisional and diabetic wound biopsies collected at different time points showed that the thickness of the epidermis and dermis, and positive picrosirius‐red stained areas for collagen, were significantly greater in the presence of hWJSCs compared with controls (P 
ISSN:0730-2312
1097-4644
DOI:10.1002/jcb.28398