Williamsia aurantiacus sp. nov. a novel actinobacterium producer of antimicrobial compounds isolated from the marine sponge

• Published in: Archives of microbiology 2019-07, Vol.201 (5), p.691-698
• Main Authors: de Menezes, Cláudia Beatriz Afonso, Afonso, Rafael Sanches, de Souza, Wallace Rafael, Parma, Márcia Maria, de Melo, Itamar Soares, Fugita, Fernando Lucas Satoru, Moraes, Luiz Alberto Beraldo, Zucchi, Tiago Domingues, Fantinatti-Garboggini, Fabiana
• Format: Article
• Language: English
• Subjects: Antibiotics; Antiinfectives and antibacterials; Antimicrobial agents; Biochemistry; Biomedical and Life Sciences; Biotechnology; Cell Biology; Deoxyribonucleic acid; DNA; Ecology; Fatty acids; Life Sciences; Low level; Microbial Ecology; Microbiology; New species; Original Paper; rRNA 16S; Strains (organisms)
• ISSN: 0302-8933; 1432-072X
• DOI: 10.1007/s00203-019-01633-z

An antibiotic-producing actinobacterium, designated isolate B375 T , was isolated from marine sponge Glodia corticostylifera collected from Praia Guaecá, São Paulo, Brazil (23°49S; 45°25W), and its taxonomic position established using data from a polyphasic study. The organism showed a combination of morphological, physiological, biochemical and chemotaxonomic characteristics consistent with its classification in the genus Williamsia . Comparative 16S rRNA gene sequence analysis indicated that the strain B375 T was most closely related to Williamsia serinedens DSM 45037 T and Williamsia spongiae DSM 46676 T and having 99.43% and 98.65% similarities, respectively, but was distinguished from these strains by a low level of DNA–DNA relatedness (53.2–63.2%) and discriminatory phenotypic properties. Chemotaxonomic investigations revealed the presence of cell-wall chemotype IV and N -glycolated muramic acid residues present in the wall cells. The cells contained C 16:0 (23.3%), C 18:0 10-methyl (23.2%) and C 18:1 ω9c (21.6%) as the major cellular fatty acids. The strain B375 T inhibited growing of Staphylococcus aureus and Colletotrichum gloeosporioides strains and was considered a producer of antimicrobial compounds. Based on the data obtained, the isolate B375 T (= CBMAI 1090 T  = DSM 46677 T ) should, therefore, be classified as the type strain of a novel species of the genus Williamsia , for which the name Williamsia aurantiacus sp. nov. is proposed.