A Structural Change in Butyrophilin upon Phosphoantigen Binding Underlies Phosphoantigen-Mediated Vγ9Vδ2 T Cell Activation

• Published in: Immunity (Cambridge, Mass.) Mass.), 2019-04, Vol.50 (4), p.1043-1053.e5
• Main Authors: Yang, Yunyun, Li, Liping, Yuan, Linjie, Zhou, Xiaoying, Duan, Jianxin, Xiao, Hongying, Cai, Ningning, Han, Shuai, Ma, Xianqiang, Liu, Weidong, Chen, Chun-Chi, Wang, Lingle, Li, Xin, Chen, Jiahuan, Kang, Ning, Chen, Jing, Shen, Zhixun, Malwal, Satish R., Liu, Wanli, Shi, Yan, Oldfield, Eric, Guo, Rey-Ting, Zhang, Yonghui
• Format: Article
• Language: English
• Subjects: allogenic cell therapy; Antigens; Binding; Biosynthesis; Butyrophilin; butyrophilin 3A1; Cell activation; cell therapy; Crystal structure; Drug development; Experiments; HMBPP; immune stimulant; Immunotherapy; inside-out signaling; Intracellular; Lymphocytes; Lymphocytes T; Malignancy; Metabolites; Microorganisms; Microscopy; Molecular dynamics; Organic chemistry; phosphoantigens; Proteins; Software; T cell antigen recognition; T cell receptors; Vγ9Vδ2 T cells
• ISSN: 1074-7613; 1097-4180
• DOI: 10.1016/j.immuni.2019.02.016

Human Vγ9Vδ2 T cells respond to microbial infections and malignancy by sensing diphosphate-containing metabolites called phosphoantigens, which bind to the intracellular domain of butyrophilin 3A1, triggering extracellular interactions with the Vγ9Vδ2 T cell receptor (TCR). Here, we examined the molecular basis of this “inside-out” triggering mechanism. Crystal structures of intracellular butyrophilin 3A proteins alone or in complex with the potent microbial phosphoantigen HMBPP or a synthetic analog revealed key features of phosphoantigens and butyrophilins required for γδ T cell activation. Analyses with chemical probes and molecular dynamic simulations demonstrated that dimerized intracellular proteins cooperate in sensing HMBPP to enhance the efficiency of γδ T cell activation. HMBPP binding to butyrophilin doubled the binding force between a γδ T cell and a target cell during “outside” signaling, as measured by single-cell force microscopy. Our findings provide insight into the “inside-out” triggering of Vγ9Vδ2 T cell activation by phosphoantigen-bound butyrophilin, facilitating immunotherapeutic drug design. [Display omitted] •The crystal structure of HMBPP-bound intracellular BTN3A1 was determined at 1.97 Å•HMBPP forms hydrogen bonds with H 351 for efficient Vγ9Vδ2 T cell activation•An asymmetric intracellular dimer is involved in HMBPP-mediated γδ T cell activation•HMBPP doubles the binding force between extracellular BTN3A and Vγ9Vδ2 TCR Vγ9Vδ2 T cells sense tumor and microbial metabolites without MHC restriction. Yang et al. used a multifaceted approach to show how the highly potent microbial HMBPP binds to BTN3A1 and triggers inside-out signaling to activate Vγ9Vδ2 T cells. This study will have implications in emerging clinical applications of allogeneic Vγ9Vδ2 T cells.