Spatially Resolved Membrane Transport in a Single Cell Imaged by Second Harmonic Light Scattering

We demonstrate that time-resolved second harmonic (SH) light scattering, when applied as an imaging modality, can be used to spatially resolve the adsorption and transport rates of molecules diffusing across the membrane in a living cell. As a representative example, we measure the passive transport...

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Bibliographic Details
Published in:Biochemistry (Easton) 2019-04, Vol.58 (14), p.1841-1844
Main Authors: Sharifian Gh, Mohammad, Wilhelm, Michael J, Moore, Michael, Dai, Hai-Lung
Format: Article
Language:English
Subjects:
Adsorption
Biological Transport
Cell Membrane - metabolism
Cells, Cultured
Dermis - cytology
Diffusion
Fibroblasts - cytology
Fibroblasts - metabolism
Humans
Reproducibility of Results
Rosaniline Dyes - chemistry
Rosaniline Dyes - metabolism
Scattering, Radiation
Second Harmonic Generation Microscopy - methods
Single-Cell Analysis - methods
Time-Lapse Imaging - methods
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Summary:We demonstrate that time-resolved second harmonic (SH) light scattering, when applied as an imaging modality, can be used to spatially resolve the adsorption and transport rates of molecules diffusing across the membrane in a living cell. As a representative example, we measure the passive transport of the amphiphilic ion, malachite green, across the plasma membrane in living human dermal fibroblast cells. Analysis of the time-resolved SH images reveals that membrane regions, which appear to be enduring higher stress, exhibit slower transport rates. It is proposed that this stress-transport relation may be a result of local enrichment of membrane rigidifiers as part of a response to maintain membrane integrity under strain.
ISSN:0006-2960
1520-4995
DOI:10.1021/acs.biochem.9b00110