Biocompatibility of a bioceramic silicone-based sealer in subcutaneous tissue

This study evaluated the biocompatibility of a new silicone-based sealer (GuttaFlow Bioseal) in rat subcutaneous tissue and compared the results with those for GuttaFlow2 and AH Plus. Each of 16 Wistar rats received four subcutaneous tissue implants, namely, GuttaFlow Bioseal, GuttaFlow2, AH Plus, a...

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Bibliographic Details
Published in:Journal of Oral Science 2019, Vol.61(1), pp.171-177
Main Authors: Santos, João M., Pereira, Sara, Sequeira, Diana B., Messias, Ana L., Martins, João B., Cunha, Henrique, Palma, Paulo J., Santos, Ana C.
Format: Article
Language:English
Subjects:
Animals
bioactive materials
bioceramic
bioceramic-based sealers
biocompatibility
Biocompatible Materials
Ceramics
Dimethylpolysiloxanes
Drug Combinations
Gutta-Percha
Rats
Rats, Wistar
Resin Cements
Root Canal Filling Materials
root canal sealer
Silicones - chemistry
subcutaneous tissue
Subcutaneous Tissue - metabolism
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Summary:This study evaluated the biocompatibility of a new silicone-based sealer (GuttaFlow Bioseal) in rat subcutaneous tissue and compared the results with those for GuttaFlow2 and AH Plus. Each of 16 Wistar rats received four subcutaneous tissue implants, namely, GuttaFlow Bioseal, GuttaFlow2, AH Plus, and one empty polyethylene tube. Eight rats were euthanized at day 8 and the remaining eight at day 30. Histological sections were stained with haematoxylin and eosin and analysed with a light microscope. Scores were established for inflammatory reaction, macrophage infiltrate, thickness of the fibrous capsule, and vascular changes. Differences between groups were assessed by using the Friedman test with Bonferroni correction. Histological analysis showed that GuttaFlow Bioseal had the lowest inflammatory reaction of all tested sealers at day 8. At day 30, the silicone-based sealers had similar inflammation profiles, but inflammation scores were nonsignificantly higher for AH Plus than for the negative control. The inflammatory reaction decreased from day 8 to day 30 in all sealers. GuttaFlow Bioseal had the most macrophage infiltrate. Under the present experimental conditions, GuttaFlow Bioseal induced limited inflammatory reactions at days 8 and 30, and initial inflammatory reactions to GuttaFlow2 and AH Plus subsided within 30 days. All tested sealers exhibited satisfactory biocompatibility at day 30 after subcutaneous implantation.
ISSN:1343-4934
1880-4926
DOI:10.2334/josnusd.18-0145