Demonstration of horizontal gene transfer from genetically engineered Thermosynechococcus elongatus BP1 to wild-type E. coli DH5α

• Published in: Gene 2019-07, Vol.704, p.49-58
• Main Authors: Nguyen, Thu H., Barnes, Cherrelle L., Agola, Jason P., Sherazi, Sana, Greene, Lesley H., Lee, James W.
• Format: Article
• Language: English
• Subjects: Bio-risk; Chromosomal integration; Cyanobacteria; E. coli; Genetically engineered cyanobacteria; Horizontal gene transfer
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/j.gene.2019.03.014

Synthetic biology with genetically engineered (GE) cyanobacteria has the potential to produce valuable products such as biofuels. However, it is also essential to assess the potential risks of synthetic biology technology before it can be widely used. In order to address key concerns posed by the application of synthetic biology to microorganisms, studies were designed to monitor the horizontal transfer of engineered genes from GE cyanobacteria Thermosynechococcus elongatus BP1 to Escherichia coli through co-incubation. The results of these experiments demonstrated that the genetically engineered DNA construct containing alcohol producing genes and kanamycin resistance can be horizontally transferred from GE T. elongatus BP1 to wild-type E. coli following two days of liquid co-culturing. The rapid and facile transfer of foreign genes, which include antibiotic resistance, between bacterial communities signifies the need to continue to deepen our understanding of the process of horizontal gene transfer, chromosomal integration as well as further biosafety-oriented research efforts. In the era of synthetic biology, the natural microbial process for sharing genetic material will also significantly impact risk assessments, containment approaches and further policy development. [Display omitted] •It is essential to assess the potential risks of synthetic biology with genetically engineered (GE) cyanobacteria.•Studies were designed to monitor the horizontal transfer of engineered genes from GE T. elongatus to E. coli.•Experiments demonstrated the horizontal transfer of transgenes from GE T. elongatus to E. coli in two days of co-culturing.