Comparative proteomics illustrates the complexity of Fe, Mn and Zn deficiency-responsive mechanisms of potato (Solanum tuberosum L.) plants in vitro

• Published in: Planta 2019-07, Vol.250 (1), p.199-217
• Main Authors: Cheng, Lixiang, Zhang, Shaomei, Yang, Lili, Wang, Yuping, Yu, Bin, Zhang, Feng
• Format: Article
• Language: English
• Subjects: Acclimation; Acclimatization; Agriculture; Antioxidants; Biomedical and Life Sciences; Biosynthesis; Cascades; Cellular structure; Cluster analysis; Clustering; Complexity; Degradation; Ecology; Electrophoresis; Electrophoresis, Gel, Two-Dimensional; Ethylenediaminetetraacetic acids; Forestry; Gel electrophoresis; Gene Regulatory Networks; Homeostasis; Image analysis; Image processing; Iron; Iron Deficiencies; Iron deficiency; Life Sciences; Manganese; Manganese - deficiency; Metabolism; Metals; Molecular modelling; ORIGINAL ARTICLE; Photosynthesis; Plant Leaves - physiology; Plant Proteins - metabolism; Plant Sciences; Plant Tubers - physiology; Plantlets; Potatoes; Protein biosynthesis; Protein deficiency; Protein turnover; Proteins; Proteome; Proteomics; Renewable energy; Signaling; Solanum tuberosum - physiology; Transcription; Two dimensional analysis; Vegetables; Zinc; Zinc - deficiency; Zinc chloride
• ISSN: 0032-0935; 1432-2048
• DOI: 10.1007/s00425-019-03163-w

Micronutrient deficiency is an important limiting factor for potato production that causes substantial tuber yield and quality losses. To under the underlying molecular mechanisms of potato in response to Fe, Mn and Zn deficiency, a comparative proteomic approach was applied. Leaf proteome change of in vitro-propagated potato plantlets subjected to a range of Fe-deficiency treatments (20, 10 and 0 µM Na-Fe-EDTA), Mn-deficiency treatments (1 and 0 µM MnCl₂·4H₂O) and Zn-deficiency treatment (0 µM ZnCl₂) using two-dimensional gel electrophoresis was analyzed. Quantitative image analysis showed a total of 146, 55 and 42 protein spots under Fe, Mn and Zn deficiency with their abundance significantly altered (P < 0.05) more than twofold, respectively. By MALDI-TOF/TOF MS analyses, the differentially abundant proteins were found mainly involved in bioenergy and metabolism, photosynthesis, defence, redox homeostasis and protein biosynthesis/degradation under the metal deficiencies. Signaling, transport, cellular structure and transcription-related proteins were also identified. The hierarchical clustering results revealed that these proteins were involved in a dynamic network in response to Fe, Mn and Zn deficiency. All these metal deficiencies caused cellular metabolic remodeling to improve metal acquisition and distribution in potato plants. The reduced photosynthetic efficiency occurred under each metal deficiency, yet Fe-deficient plants showed a more severe damage of photosynthesis. More defence mechanisms were induced by Fe deficiency than Mn and Zn deficiency, and the antioxidant systems showed different responses to each metal deficiency. Reprogramming of protein biosynthesis/degradation and assembly was more strongly required for acclimation to Fe deficiency. The signaling cascades involving auxin and NDPKs might also play roles in micronutrient stress signaling and pinpoint interesting candidates for future studies. Our results first provide an insight into the complex functional and regulatory networks in potato plants under Fe, Mn and Zn deficiency.