Design and Demonstration of Tunable Amplified Sensitivity of AlGaN/GaN High Electron Mobility Transistor (HEMT)-Based Biosensors in Human Serum

We have developed a swift and simplistic protein immunoassay using aptamer functionalized AlGaN/GaN high electron mobility transistors (HEMTs). The unique design of the sensor facilitates protein detection in a physiological salt environment overcoming charge screening effects, without requiring sam...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2019-05, Vol.91 (9), p.5953-5960
Main Authors: Tai, Tse-Yu, Sinha, Anirban, Sarangadharan, Indu, Pulikkathodi, Anil Kumar, Wang, Shin-Li, Lee, Geng-Yen, Chyi, Jen-Inn, Shiesh, Shu-Chu, Lee, Gwo-Bin, Wang, Yu-Lin
Format: Article
Language:English
Subjects:
Aluminum gallium nitrides
Amplification
Analytical chemistry
Aptamers
Biomarkers
Biosensors
Chemistry
Congestive heart failure
Electric double layer
Environmental effects
Field effect transistors
High electron mobility transistors
Immunoassay
Ionic strength
Marine environment
Mobility
Physiological effects
Proteins
Selectivity
Semiconductor devices
Sensitivity
Sensitivity enhancement
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Summary:We have developed a swift and simplistic protein immunoassay using aptamer functionalized AlGaN/GaN high electron mobility transistors (HEMTs). The unique design of the sensor facilitates protein detection in a physiological salt environment overcoming charge screening effects, without requiring sample preprocessing. This study reports a tunable and amplified sensitivity of solution-gated electric double layer (EDL) HEMT-based biosensors, which demonstrates significantly enhanced sensitivity by designing a smaller gap between the gate electrode and the detection, and by operating at higher gate voltage. Sensitivity is calculated by quantifying NT-proBNP, a clinical biomarker of heart failure, in buffer and untreated human serum samples. The biosensor depicts elevated sensitivity and high selectivity. Furthermore, detailed investigation of the amplified sensitivity in an increased ionic strength environment is conducted, and it is revealed that a high sensitivity of 80.54 mV/decade protein concentration can be achieved, which is much higher than that of previously reported FET biosensors. This sensor technology demonstrates immense potential in developing surface affinity sensors for clinical diagnostics.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.9b00353