Immunological evaluation of pasteurized, deaerated human milk

Retention of immunoglobulin A (IgA) and IgA specific for enteropathogenic Escherichia coli (EPEC) was determined in human milk that was processed and stored under refrigeration or frozen conditions. Human milk was subjected to three different processing conditions: (a) deaerated, vacuum packaged in...

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Bibliographic Details
Published in:Journal of food protection 1990-11, Vol.53 (11), p.975-977
Main Authors: Musgrove, M.T. (University of Georgia, Athens, GA), Harrison, M.A, Eitenmiller, R.R
Format: Article
Language:English
Subjects:
Biological and medical sciences
ELISA
EMBALAJE EN VACIO
EMBALLAGE SOUS VIDE
Food industries
Food microbiology
Fundamental and applied biological sciences. Psychology
IMMUNOGLOBULINE
INMUNOGLOBULINA
LAIT HUMAIN
LECHE HUMANA
OXIGENO
OXYGENE
PASTEURISATION
PASTEURIZACION
TECHNOLOGIE ALIMENTAIRE
TECNOLOGIA DE LOS ALIMENTOS
TEMPERATURA
TEMPERATURE
TEST ELISA
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Summary:Retention of immunoglobulin A (IgA) and IgA specific for enteropathogenic Escherichia coli (EPEC) was determined in human milk that was processed and stored under refrigeration or frozen conditions. Human milk was subjected to three different processing conditions: (a) deaerated, vacuum packaged in metalized polyester bags and pasteurized (64 degree C for 6 min, 10 s); (b) vacuum packaged and pasteurized; (c) vacuum packaged. Samples were stored at 4 degree C for 7 d and at -20 degree C for 28 d. An enzyme-linked immunosorbent assay (ELISA) to detect antibodies to EPEC was developed and used to monitor these antibody levels, while radial immunodiffusion (RID) plates were used to provide information on total IgA of the milk samples. In addition, the dissolved oxygen content of the samples was monitored. It was found that pasteurization and deaeration did not adversely affect the IgA levels, and levels of total IgA and IgA specific for EPEC remained stable or increased slightly during storage in most samples regardless of the storage temperatures
ISSN:0362-028X
1944-9097
DOI:10.4315/0362-028X-53.11.975