Emergence of two novel recombinant porcine reproductive and respiratory syndrome viruses 2 (lineage 3) in Southwestern China

• Published in: Veterinary microbiology 2019-05, Vol.232, p.30-41
• Main Authors: Zhou, Long, Kang, Runmin, Zhang, Yi, Yu, Jifeng, Xie, Bo, Chen, Changying, Li, Xingyu, Chen, Bin, Liang, Luqi, Zhu, Jiawen, Tian, Yiming, Yang, Xin, Wang, Hongning
• Format: Article
• Language: English
• Subjects: 3' Untranslated regions; Amino acid sequence; Genomes; Genotyping; Homology; Lineage 3; Nucleotide sequence; ORF5; Pathogenicity; Pathogens; Phylogeny; Porcine reproductive and respiratory syndrome virus (PRRSV); Recombination; Strains (organisms); Swine; Virulence; Viruses
• ISSN: 0378-1135; 1873-2542
• DOI: 10.1016/j.vetmic.2019.01.026

•The genomic characterization of two novel lineage 3 PRRSVs (GZgy17 and SCya18) was analyzed.•Multiple novel aa/nt deletions were observed within the genomes of GZgy17 and SCya18.•GZgy17 and SCya18 both originated from a lineage 3 strain and had recombined with lineage 8 and 1 strain, respectively.•GZgy17 exhibited higher pathogenicity in piglets compared to SCya18. The lineage 3 of porcine reproductive and respiratory syndrome virus 2 (PRRSV-2) was first reported in mainland China in 2010 and it has spread rapidly in recent years. Here, two novel lineage 3 strains of PRRSV-2 were isolated from diseased pigs in Southwestern China during 2017–2018, and were designated as GZgy17 and SCya18. The complete genomes of the two isolates were then determined, and sequence alignment revealed that GZgy17 had the same discontinuous 30-amino acid (aa) deletion in NSP2 as JXA1, while SCya18 contained the discontinuous 131-aa deletion in NSP2 identical to that of NADC30, when compared to the strain VR-2332. Notably, GZgy17 contained an additional 19-aa deletion in NSP2, and SCya18 had a unique 3-nt deletion in its 3’UTR. Homology and phylogenetic analysis showed that GZgy17 and SCya18 shared low nucleotide homology (91.2–92.0%) with QYYZ and were classified into a new cluster of lineage 3 strains based on ORF5 genotyping. Recombination analyses revealed that GZgy17 and SCya18 both originated from a SH/CH/2016-like (lineage 3) strain and had recombined with a JXA1-like (lineage 8) and a NADC30-like (lineage 1) strain, respectively. Furthermore, we compared the virulence of the two strains in 4-week-old piglets. The results showed that GZgy17 caused mortality rates of 20% and exhibited higher pathogenicity in piglets compared to SCya18. Our findings suggest that recombination might be responsible for the variations in pathogenicity of lineage 3 strains of PRRSV-2 and highlight the importance of surveillance of this lineage in China.