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Conductive hydrogel based on chitosan-aniline pentamer/gelatin/agarose significantly promoted motor neuron-like cells differentiation of human olfactory ecto-mesenchymal stem cells
Developing a simple produces for efficient derivation of motor neurons (MNs) is essential for neural tissue engineering studies. Stem cells with high capacity for neural differentiation and scaffolds with the potential to promote motor neurons differentiation are promising candidates for neural tiss...
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Published in: | Materials Science & Engineering C 2019-08, Vol.101, p.243-253 |
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creator | Bagher, Zohreh Atoufi, Zhaleh Alizadeh, Rafieh Farhadi, Mohammad Zarrintaj, Payam Moroni, Lorenzo Setayeshmehr, Mohsen Komeili, Ali Kamrava, S. Kamran |
description | Developing a simple produces for efficient derivation of motor neurons (MNs) is essential for neural tissue engineering studies. Stem cells with high capacity for neural differentiation and scaffolds with the potential to promote motor neurons differentiation are promising candidates for neural tissue engineering. Recently, human olfactory ecto-mesenchymal stem cells (OE-MSCs), which are isolated easily from the olfactory mucosa, are considered a new hope for neuronal replacement due to their neural crest origin. Herein, we synthesized conducting hydrogels using different concentration of chitosan-g-aniline pentamer, gelatin, and agarose. The chemical structures, swelling and deswelling ratio, ionic conductivity and thermal properties of the hydrogel were characterized. Scaffolds with 10% chitosan-g-aniline pentamer/gelatin (S10) were chosen for further investigation and the potential of OE-MSCs as a new source for programming to motor neuron-like cells investigated on tissue culture plate (TCP) and conductive hydrogels. Cell differentiation was evaluated at the level of mRNA and protein synthesis and indicated that conductive hydrogels significantly increased the markers related to motor neurons including Hb-9, Islet-1 and ChAT compared to TCP. Taken together, the results suggest that OE-MSCs would be successfully differentiated into motor neuron-like cells on conductive hydrogels and would have a promising potential for treating motor neuron-related diseases.
[Display omitted]
•Olfactory ecto-mesenchymal stem cells (OE-MSCs) have tendency to give rise to motor neuron.•Conductive hydrogels has the potential to enhance the motor neuron-like cells differentiation.•Conductive scaffolds containing OE-MSCs holds a new hope for treatment of motor neuron diseases. |
doi_str_mv | 10.1016/j.msec.2019.03.068 |
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[Display omitted]
•Olfactory ecto-mesenchymal stem cells (OE-MSCs) have tendency to give rise to motor neuron.•Conductive hydrogels has the potential to enhance the motor neuron-like cells differentiation.•Conductive scaffolds containing OE-MSCs holds a new hope for treatment of motor neuron diseases.</description><identifier>ISSN: 0928-4931</identifier><identifier>EISSN: 1873-0191</identifier><identifier>DOI: 10.1016/j.msec.2019.03.068</identifier><identifier>PMID: 31029317</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Aniline ; Aniline Compounds - chemical synthesis ; Aniline Compounds - pharmacology ; Aniline pentamer ; Calcium Phosphates - pharmacology ; Cell culture ; Cell differentiation ; Cell Differentiation - drug effects ; Cell Proliferation - drug effects ; Cell Survival - drug effects ; Cells, Cultured ; Chitosan ; Chitosan - chemical synthesis ; Chitosan - chemistry ; Chitosan - pharmacology ; Compressive Strength ; Conductive polymers ; Differentiation (biology) ; Electric Conductivity ; Gelatin ; Gelatin - chemistry ; Gelatin - pharmacology ; Humans ; Hydrogels ; Hydrogels - chemistry ; Hydrogels - pharmacology ; Ion currents ; Islet-1 protein ; Male ; Materials science ; Mesenchymal stem cells ; Mesenchymal Stem Cells - cytology ; Mesenchymal Stem Cells - drug effects ; Mesenchymal Stem Cells - ultrastructure ; Mesenchyme ; Motor neuron differentiation ; Motor neurons ; Motor Neurons - cytology ; Motor Neurons - drug effects ; mRNA ; Mucosa ; Nasal ectomesenchymal stem cells ; Neural crest ; Neurons ; Olfactory Bulb - cytology ; Olfactory epithelium ; Organic chemistry ; Plates (structural members) ; Protein biosynthesis ; Protein synthesis ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Scaffolds ; Sepharose - pharmacology ; Spectroscopy, Fourier Transform Infrared ; Stem cells ; Temperature ; Thermal properties ; Thermodynamic properties ; Thermogravimetry ; Tissue culture ; Tissue engineering ; Tissue Scaffolds - chemistry ; Transcription</subject><ispartof>Materials Science & Engineering C, 2019-08, Vol.101, p.243-253</ispartof><rights>2019</rights><rights>Copyright © 2019. Published by Elsevier B.V.</rights><rights>Copyright Elsevier BV Aug 2019</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c465t-6bb2777164ce891a6cde00026bfb5a323012e1832a5e8cb7049c1fb90ccd2fe93</citedby><cites>FETCH-LOGICAL-c465t-6bb2777164ce891a6cde00026bfb5a323012e1832a5e8cb7049c1fb90ccd2fe93</cites><orcidid>0000-0003-1298-6025</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31029317$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bagher, Zohreh</creatorcontrib><creatorcontrib>Atoufi, Zhaleh</creatorcontrib><creatorcontrib>Alizadeh, Rafieh</creatorcontrib><creatorcontrib>Farhadi, Mohammad</creatorcontrib><creatorcontrib>Zarrintaj, Payam</creatorcontrib><creatorcontrib>Moroni, Lorenzo</creatorcontrib><creatorcontrib>Setayeshmehr, Mohsen</creatorcontrib><creatorcontrib>Komeili, Ali</creatorcontrib><creatorcontrib>Kamrava, S. Kamran</creatorcontrib><title>Conductive hydrogel based on chitosan-aniline pentamer/gelatin/agarose significantly promoted motor neuron-like cells differentiation of human olfactory ecto-mesenchymal stem cells</title><title>Materials Science & Engineering C</title><addtitle>Mater Sci Eng C Mater Biol Appl</addtitle><description>Developing a simple produces for efficient derivation of motor neurons (MNs) is essential for neural tissue engineering studies. Stem cells with high capacity for neural differentiation and scaffolds with the potential to promote motor neurons differentiation are promising candidates for neural tissue engineering. Recently, human olfactory ecto-mesenchymal stem cells (OE-MSCs), which are isolated easily from the olfactory mucosa, are considered a new hope for neuronal replacement due to their neural crest origin. Herein, we synthesized conducting hydrogels using different concentration of chitosan-g-aniline pentamer, gelatin, and agarose. The chemical structures, swelling and deswelling ratio, ionic conductivity and thermal properties of the hydrogel were characterized. Scaffolds with 10% chitosan-g-aniline pentamer/gelatin (S10) were chosen for further investigation and the potential of OE-MSCs as a new source for programming to motor neuron-like cells investigated on tissue culture plate (TCP) and conductive hydrogels. Cell differentiation was evaluated at the level of mRNA and protein synthesis and indicated that conductive hydrogels significantly increased the markers related to motor neurons including Hb-9, Islet-1 and ChAT compared to TCP. Taken together, the results suggest that OE-MSCs would be successfully differentiated into motor neuron-like cells on conductive hydrogels and would have a promising potential for treating motor neuron-related diseases.
[Display omitted]
•Olfactory ecto-mesenchymal stem cells (OE-MSCs) have tendency to give rise to motor neuron.•Conductive hydrogels has the potential to enhance the motor neuron-like cells differentiation.•Conductive scaffolds containing OE-MSCs holds a new hope for treatment of motor neuron diseases.</description><subject>Aniline</subject><subject>Aniline Compounds - chemical synthesis</subject><subject>Aniline Compounds - pharmacology</subject><subject>Aniline pentamer</subject><subject>Calcium Phosphates - pharmacology</subject><subject>Cell culture</subject><subject>Cell differentiation</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Proliferation - drug effects</subject><subject>Cell Survival - drug effects</subject><subject>Cells, Cultured</subject><subject>Chitosan</subject><subject>Chitosan - chemical synthesis</subject><subject>Chitosan - chemistry</subject><subject>Chitosan - pharmacology</subject><subject>Compressive Strength</subject><subject>Conductive polymers</subject><subject>Differentiation (biology)</subject><subject>Electric Conductivity</subject><subject>Gelatin</subject><subject>Gelatin - chemistry</subject><subject>Gelatin - pharmacology</subject><subject>Humans</subject><subject>Hydrogels</subject><subject>Hydrogels - chemistry</subject><subject>Hydrogels - pharmacology</subject><subject>Ion currents</subject><subject>Islet-1 protein</subject><subject>Male</subject><subject>Materials science</subject><subject>Mesenchymal stem cells</subject><subject>Mesenchymal Stem Cells - cytology</subject><subject>Mesenchymal Stem Cells - drug effects</subject><subject>Mesenchymal Stem Cells - ultrastructure</subject><subject>Mesenchyme</subject><subject>Motor neuron differentiation</subject><subject>Motor neurons</subject><subject>Motor Neurons - cytology</subject><subject>Motor Neurons - drug effects</subject><subject>mRNA</subject><subject>Mucosa</subject><subject>Nasal ectomesenchymal stem cells</subject><subject>Neural crest</subject><subject>Neurons</subject><subject>Olfactory Bulb - cytology</subject><subject>Olfactory epithelium</subject><subject>Organic chemistry</subject><subject>Plates (structural members)</subject><subject>Protein biosynthesis</subject><subject>Protein synthesis</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Scaffolds</subject><subject>Sepharose - pharmacology</subject><subject>Spectroscopy, Fourier Transform Infrared</subject><subject>Stem cells</subject><subject>Temperature</subject><subject>Thermal properties</subject><subject>Thermodynamic properties</subject><subject>Thermogravimetry</subject><subject>Tissue culture</subject><subject>Tissue engineering</subject><subject>Tissue Scaffolds - chemistry</subject><subject>Transcription</subject><issn>0928-4931</issn><issn>1873-0191</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp9kc-O0zAQxi0EYkvhBTggS1y4JPWfNE4kLqhaWKSVuMDZcpxJ6xLbxU5WynvxgEzUhQOHvXis0W8-j7-PkLeclZzxencufQZbCsbbksmS1c0zsuGNkgV2-HOyYa1oiqqV_Ia8yvnMkJBKvCQ3kjOBbbUhvw8x9LOd3APQ09KneISRdiZDT2Og9uSmmE0oTHCjC0AvECbjIe0QM5MLO3M0KWag2R2DG5w1YRoXeknRxwk18IyJBphTDMXofgK1MI6Z9m4YIKGYQxV8KA70NHuDl3EwFmcWClgKDxmCPS3ejDRP4K_jr8mLwYwZ3jzWLfnx-fb74a64__bl6-HTfWGrej8VddcJpRSvKwtNy01te2CMibobur2RQjIugDdSmD00tlOsai0fupZZ24sBWrklH666-J9fM-RJe5fXDUyAOGctBK-VEkpyRN__h57jnAJuh1RV1ZWqlEBKXCmLpuUEg74k501aNGd6zVSf9ZqpXjPVTOo1sS159yg9dx76fyN_Q0Tg4xUA9OLBQdLZOrQNepfQRd1H95T-H2H_uAg</recordid><startdate>20190801</startdate><enddate>20190801</enddate><creator>Bagher, Zohreh</creator><creator>Atoufi, Zhaleh</creator><creator>Alizadeh, Rafieh</creator><creator>Farhadi, Mohammad</creator><creator>Zarrintaj, Payam</creator><creator>Moroni, Lorenzo</creator><creator>Setayeshmehr, Mohsen</creator><creator>Komeili, Ali</creator><creator>Kamrava, S. 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Kamran</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Conductive hydrogel based on chitosan-aniline pentamer/gelatin/agarose significantly promoted motor neuron-like cells differentiation of human olfactory ecto-mesenchymal stem cells</atitle><jtitle>Materials Science & Engineering C</jtitle><addtitle>Mater Sci Eng C Mater Biol Appl</addtitle><date>2019-08-01</date><risdate>2019</risdate><volume>101</volume><spage>243</spage><epage>253</epage><pages>243-253</pages><issn>0928-4931</issn><eissn>1873-0191</eissn><abstract>Developing a simple produces for efficient derivation of motor neurons (MNs) is essential for neural tissue engineering studies. Stem cells with high capacity for neural differentiation and scaffolds with the potential to promote motor neurons differentiation are promising candidates for neural tissue engineering. Recently, human olfactory ecto-mesenchymal stem cells (OE-MSCs), which are isolated easily from the olfactory mucosa, are considered a new hope for neuronal replacement due to their neural crest origin. Herein, we synthesized conducting hydrogels using different concentration of chitosan-g-aniline pentamer, gelatin, and agarose. The chemical structures, swelling and deswelling ratio, ionic conductivity and thermal properties of the hydrogel were characterized. Scaffolds with 10% chitosan-g-aniline pentamer/gelatin (S10) were chosen for further investigation and the potential of OE-MSCs as a new source for programming to motor neuron-like cells investigated on tissue culture plate (TCP) and conductive hydrogels. Cell differentiation was evaluated at the level of mRNA and protein synthesis and indicated that conductive hydrogels significantly increased the markers related to motor neurons including Hb-9, Islet-1 and ChAT compared to TCP. Taken together, the results suggest that OE-MSCs would be successfully differentiated into motor neuron-like cells on conductive hydrogels and would have a promising potential for treating motor neuron-related diseases.
[Display omitted]
•Olfactory ecto-mesenchymal stem cells (OE-MSCs) have tendency to give rise to motor neuron.•Conductive hydrogels has the potential to enhance the motor neuron-like cells differentiation.•Conductive scaffolds containing OE-MSCs holds a new hope for treatment of motor neuron diseases.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>31029317</pmid><doi>10.1016/j.msec.2019.03.068</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0003-1298-6025</orcidid><oa>free_for_read</oa></addata></record> |
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source | ScienceDirect Journals |
subjects | Aniline Aniline Compounds - chemical synthesis Aniline Compounds - pharmacology Aniline pentamer Calcium Phosphates - pharmacology Cell culture Cell differentiation Cell Differentiation - drug effects Cell Proliferation - drug effects Cell Survival - drug effects Cells, Cultured Chitosan Chitosan - chemical synthesis Chitosan - chemistry Chitosan - pharmacology Compressive Strength Conductive polymers Differentiation (biology) Electric Conductivity Gelatin Gelatin - chemistry Gelatin - pharmacology Humans Hydrogels Hydrogels - chemistry Hydrogels - pharmacology Ion currents Islet-1 protein Male Materials science Mesenchymal stem cells Mesenchymal Stem Cells - cytology Mesenchymal Stem Cells - drug effects Mesenchymal Stem Cells - ultrastructure Mesenchyme Motor neuron differentiation Motor neurons Motor Neurons - cytology Motor Neurons - drug effects mRNA Mucosa Nasal ectomesenchymal stem cells Neural crest Neurons Olfactory Bulb - cytology Olfactory epithelium Organic chemistry Plates (structural members) Protein biosynthesis Protein synthesis RNA, Messenger - genetics RNA, Messenger - metabolism Scaffolds Sepharose - pharmacology Spectroscopy, Fourier Transform Infrared Stem cells Temperature Thermal properties Thermodynamic properties Thermogravimetry Tissue culture Tissue engineering Tissue Scaffolds - chemistry Transcription |
title | Conductive hydrogel based on chitosan-aniline pentamer/gelatin/agarose significantly promoted motor neuron-like cells differentiation of human olfactory ecto-mesenchymal stem cells |
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