EB1‐ and EB2‐dependent anterograde trafficking of TRPM4 regulates focal adhesion turnover and cell invasion

• Published in: The FASEB journal 2019-08, Vol.33 (8), p.9434-9452
• Main Authors: Blanco, Constanza, Morales, Danna, Mogollones, Ignacio, Vergara‐Jaque, Ariela, Vargas, Carla, Álvarez, Alhejandra, Riquelme, Denise, Leiva‐Salcedo, Elías, González, Wendy, Morales, Diego, Maureira, Diego, Aldunate, Ismael, Cáceres, Mónica, Varela, Diego, Cerda, Oscar
• Format: Article
• Language: English
• Subjects: EB proteins; ER export; TRP channels
• ISSN: 0892-6638; 1530-6860
• DOI: 10.1096/fj.201900136R

ABSTRACT Transient receptor potential melastatin 4 (TRPM4) is a Ca2+‐activated nonselective cationic channel involved in a wide variety of physiologic and pathophysiological processes. Bioinformatics analyses of the primary sequence of TRPM4 allowed us to identify a putative motif for interaction with end‐binding (EB) proteins, which are microtubule plus‐end tracking proteins. Here, we provide novel data suggesting that TRPM4 interacts with EB proteins. We show that mutations of the putative EB binding motif abolish the TRPM4‐EB interaction, leading to a reduced expression of the mature population of the plasma membrane channel and instead display an endoplasmic reticulum‐associated distribution. Furthermore, we demonstrate that EB1 and EB2 proteins are required for TRPM4 trafficking and functional activity. Finally, we demonstrated that the expression of a soluble fragment containing the EB binding motif of TRPM4 reduces the plasma membrane expression of the channel and affects TRPM4‐dependent focal adhesion disassembly and cell invasion processes.—Blanco, C., Morales, D., Mogollones, I., Vergara‐Jaque, A., Vargas, C., Álvarez, A., Riquelme, D., Leiva‐Salcedo, E., González, W., Morales, D., Maureira, D., Aldunate, I., Cáceres, M., Varela, D., Cerda, O. EB1‐ and EB2‐dependent anterograde trafficking of TRPM4 regulates focal adhesion turnover and cell invasion. FASEB J. 33, 9434–9452 (2019). www.fasebj.org